PUBLICATION
Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response
- Authors
- Shan, S., Liu, R., Feng, H., Meng, F., Aizaz, M., Yang, G.
- ID
- ZDB-PUB-210705-1
- Date
- 2021
- Source
- Veterinary research 52: 88 (Journal)
- Registered Authors
- Keywords
- Cyprinus carpio L., Ifn, Innate immunity, Signaling pathway, Toll-like receptor 19 (Tlr19)
- MeSH Terms
-
- Adaptor Proteins, Vesicular Transport/metabolism
- Aeromonas hydrophila
- Animals
- Carps/genetics*
- Carps/immunology*
- Fish Diseases/immunology*
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Gene Expression Profiling/veterinary
- Gene Expression Regulation/immunology*
- Gram-Negative Bacterial Infections
- Immunity, Innate/genetics*
- Interferons/genetics
- Phylogeny
- Poly I-C
- Sequence Analysis, DNA/veterinary
- Signal Transduction
- Toll-Like Receptors/chemistry
- Toll-Like Receptors/genetics*
- Toll-Like Receptors/immunology*
- PubMed
- 34130754 Full text @ Vet. Res.
Citation
Shan, S., Liu, R., Feng, H., Meng, F., Aizaz, M., Yang, G. (2021) Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response. Veterinary research. 52:88.
Abstract
Toll-like receptor 19 (Tlr19) is a fish-specific TLR that plays a critical role in innate immunity. In the present study, we aimed to identify tlr19 from common carp (Cyprinus carpio L.) and explored its expression profile, localization, adaptor, and signaling pathways. A novel tlr19 cDNA sequence (Cctlr19) was identified in common carp. Phylogenetic analysis revealed that CcTlr19 was most closely related to Danio rerio Tlr19. Subcellular localization analysis indicates that CcTlr19 was synthesized in the free ribosome and then transported to early endosomes. Cctlr19 was constitutively expressed in all the examined tissues, with the highest expression in the brain. After poly(I:C) and Aeromonas hydrophila injection, the expression of Cctlr19 was significantly upregulated in immune-related organs. In addition, the expression of Cctlr19 was upregulated in head kidney leukocytes (HKL) upon stimulation with different ligands. Immunofluorescence and luciferase analyses indicate that CcTlr19 recruited TRIF as an adaptor. Furthermore, CcTlr19 can activate the expression of ifn-1 and viperin. Taken together, these findings lay the foundation for future research to investigate the mechanisms underlying fish tlr19.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping