PUBLICATION
The probiotic Lactobacillus rhamnosus mimics the dark-driven regulation of appetite markers and melatonin receptors' expression in zebrafish (Danio rerio) larvae: Understanding the role of the gut microbiome
- Authors
- Lutfi, E., Basili, D., Falcinelli, S., Morillas, L., Carnevali, O., Capilla, E., Navarro, I.
- ID
- ZDB-PUB-210616-3
- Date
- 2021
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 256: 110634 (Journal)
- Registered Authors
- Carnevali, Oliana
- Keywords
- Circadian rhythms, Leptin, Melatonin, Microbiota, Photoperiod
- MeSH Terms
-
- Animals
- Appetite/drug effects
- Appetite/physiology*
- Gastrointestinal Microbiome*
- Lacticaseibacillus rhamnosus/chemistry*
- Larva/drug effects
- Larva/growth & development
- Larva/metabolism*
- Melatonin/metabolism
- Photoperiod*
- Probiotics/pharmacology*
- Receptors, Melatonin/genetics
- Receptors, Melatonin/metabolism*
- Zebrafish
- PubMed
- 34119649 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Lutfi, E., Basili, D., Falcinelli, S., Morillas, L., Carnevali, O., Capilla, E., Navarro, I. (2021) The probiotic Lactobacillus rhamnosus mimics the dark-driven regulation of appetite markers and melatonin receptors' expression in zebrafish (Danio rerio) larvae: Understanding the role of the gut microbiome. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 256:110634.
Abstract
The use of probiotics has been recently considered a novel therapeutic strategy to prevent pathologies such as obesity; however, the specific mechanisms of action by which probiotics exert their beneficial effects on metabolic health remain unclear. The aim of the present study was to investigate the short-term effects of a probiotic Lactobacillus rhamnosus supplementation (PROB) on appetite regulation, growth-related markers, and microbiota diversity in zebrafish (Danio rerio) larvae, compared to a group subjected to a constant darkness photoperiod (DARK), as well as to evaluate the effects of both treatments on melatonin receptors' expression. After a 24 h treatment, both PROB and DARK conditions caused a significant increase in leptin a expression, and DARK showed a similar tendency as PROB increasing the mRNA levels of proopiomelanocortin a, supporting a negative regulation of appetite markers by these treatments. Moreover, both PROB and DARK also enhanced melatonin receptors transcript (melatonin receptor 1 ba and bb) and protein (melatonin receptor 1) abundances suggesting a potential involvement of melatonin in in mediating these effects. Nevertheless, treatments did not exhibit a significant effect on the expression of most of the growth hormone/insulin-like growth factor axis genes evaluated. Finally, only the DARK condition significantly modulated gut microbiota diversity at such short time, altogether highlighting the rapid effects of this probiotic on modulating appetite regulatory and melatonin receptors' expression, without a concomitant variation of gut microbiota.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping