PUBLICATION
Polystyrene microplastics induce apoptosis via ROS-mediated p53 signaling pathway in zebrafish
- Authors
- Umamaheswari, S., Priyadarshinee, S., Kadirvelu, K., Ramesh, M.
- ID
- ZDB-PUB-210616-22
- Date
- 2021
- Source
- Chemico-biological interactions 345: 109550 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Enzymes, Genes, Histology, Inflammation, Plastics
- MeSH Terms
-
- Animals
- Apoptosis/drug effects*
- Dose-Response Relationship, Drug
- Ecotoxicology
- Microplastics/toxicity*
- Polystyrenes/chemistry*
- Polystyrenes/toxicity*
- Reactive Oxygen Species/metabolism
- Signal Transduction/drug effects*
- Time Factors
- Tumor Suppressor Protein p53/metabolism*
- Zebrafish*
- PubMed
- 34126101 Full text @ Chem. Biol. Interact.
- CTD
- 34126101
Citation
Umamaheswari, S., Priyadarshinee, S., Kadirvelu, K., Ramesh, M. (2021) Polystyrene microplastics induce apoptosis via ROS-mediated p53 signaling pathway in zebrafish. Chemico-biological interactions. 345:109550.
Abstract
Microplastic (MP) pollution is ubiquitous and has become an emerging threat to aquatic biota. Recent scientific reports have recorded their toxic impacts at the cellular and organism levels, but the underlying molecular mechanism of their toxicity remains unclear. The present study elucidates an array of molecular events underlying apoptosis in the gills of polystyrene microplastics (PS-MPs) exposed zebrafish (Danio rerio). PS-MPs at different concentrations (10 and 100 μg L-1) induced the reactive oxygen species (ROS) generation, in turn affecting the oxidative and immune defense mechanism. The expression profile of antioxidant genes cat, sod1, gpx1a and gstp1 were altered significantly. PS-MPs also significantly inhibited the neurotransmission in zebrafish. In addition, the PS-MPs exposure upregulated the expression of p53, gadd45ba, and casp3b resulting in apoptosis. We demonstrate that PS-MPs significantly upregulate the transcriptional pattern of tnfa and ptgs2a which are essential gene markers in inflammatory mechanism. Further, the oxidative damage induced by PS-MPs exposure could lead to cytological damage resulting in altered lamellar structures, capillary dilation, and necrosis in gill histomaps. In conclusion, the findings of this work strongly suggest that PS-MPs induces dose-and time-dependent ROS mediated apoptotic responses in zebrafish. Furthermore, the physiological responses observed in the gills correlate with the above observations and helps in unravelling the potential molecular mechanism underpinning the PS-MPs toxicity in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping