PUBLICATION
Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation
- Authors
- Carbonare, L.D., Bertacco, J., Marchetto, G., Cheri, S., Deiana, M., Minoia, A., Tiso, N., Mottes, M., Valenti, M.T.
- ID
- ZDB-PUB-210608-2
- Date
- 2021
- Source
- Stem Cell Research & Therapy 12: 326 (Journal)
- Registered Authors
- Keywords
- Chondrocytes, Mesenchymal stem cells, Methylsulfonylmethane, Osteoblasts
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cells, Cultured
- Chondrocytes
- Chondrogenesis*
- Dimethyl Sulfoxide/pharmacology
- Humans
- Osteoblasts
- Osteogenesis
- Sulfones
- Zebrafish*
- PubMed
- 34090529 Full text @ Stem Cell Res. Ther.
Citation
Carbonare, L.D., Bertacco, J., Marchetto, G., Cheri, S., Deiana, M., Minoia, A., Tiso, N., Mottes, M., Valenti, M.T. (2021) Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation. Stem Cell Research & Therapy. 12:326.
Abstract
Background Methylsulfonylmethane (MSM) is a nutraceutical compound which has been indicated to counteract osteoarthritis, a cartilage degenerative disorder. In addition, MSM has also been shown to increase osteoblast differentiation. So far, few studies have investigated MSM role in the differentiation of mesenchymal stem cells (MSCs), and no study has been performed to evaluate its overall effects on both osteogenic and chondrogenic differentiation. These two mutually regulated processes share the same progenitor cells.
Methods Therefore, with the aim to evaluate the effects of MSM on chondrogenesis and osteogenesis, we analyzed the expression of SOX9, RUNX2, and SP7 transcription factors in vitro (mesenchymal stem cells and chondrocytes cell lines) and in vivo (zebrafish model). Real-time PCR as well Western blotting, immunofluorescence, and specific in vitro and in vivo staining have been performed. Student's paired t test was used to compare the variation between the groups.
Results Our data demonstrated that MSM modulates the expression of differentiation-related genes both in vitro and in vivo. The increased SOX9 expression suggests that MSM promotes chondrogenesis in treated samples. In addition, RUNX2 expression was not particularly affected by MSM while SP7 expression increased in all MSM samples/model analyzed. As SP7 is required for the final commitment of progenitors to preosteoblasts, our data suggest a role of MSM in promoting preosteoblast formation. In addition, we observed a reduced expression of the osteoclast-surface receptor RANK in larvae and in scales as well as a reduced pERK/ERK ratio in fin and scale of MSM treated zebrafish.
Conclusions In conclusion, our study provides new insights into MSM mode of action and suggests that MSM is a useful tool to counteract skeletal degenerative diseases by targeting MSC commitment and differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping