PUBLICATION
Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish
- Authors
- Orso, G., Solovyev, M.M., Facchiano, S., Tyrikova, E., Sateriale, D., Kashinskaya, E., Pagliarulo, C., Hoseinifar, H.S., Simonov, E., Varricchio, E., Paolucci, M., Imperatore, R.
- ID
- ZDB-PUB-210603-25
- Date
- 2021
- Source
- Animals : an open access journal from MDPI 11(6): (Journal)
- Registered Authors
- Keywords
- cytokines, intestinal inflammation, microbiota, polyphenols, prebiotics, zebrafish
- MeSH Terms
- none
- PubMed
- 34070355 Full text @ Animals (Basel)
Citation
Orso, G., Solovyev, M.M., Facchiano, S., Tyrikova, E., Sateriale, D., Kashinskaya, E., Pagliarulo, C., Hoseinifar, H.S., Simonov, E., Varricchio, E., Paolucci, M., Imperatore, R. (2021) Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish. Animals : an open access journal from MDPI. 11(6):.
Abstract
The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (Castanea sativa) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (Danio rerio) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (Enterobacteriaceae and Pseudomonas), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping