PUBLICATION
Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
- Authors
- Chalin, D., Bureau, C., Parmeggiani, A., Rochal, S., Kissa, K., Golushko, I.
- ID
- ZDB-PUB-210501-46
- Date
- 2021
- Source
- Scientific Reports 11: 9316 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Aorta/diagnostic imaging
- Aorta/embryology
- Aorta/physiology*
- Hematopoiesis*
- Hematopoietic Stem Cells/physiology*
- Models, Cardiovascular*
- Stress, Mechanical
- Time-Lapse Imaging
- Zebrafish
- PubMed
- 33927284 Full text @ Sci. Rep.
Citation
Chalin, D., Bureau, C., Parmeggiani, A., Rochal, S., Kissa, K., Golushko, I. (2021) Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis. Scientific Reports. 11:9316.
Abstract
All blood cells originate from hematopoietic stem/progenitor cells (HSPCs). HSPCs are formed from endothelial cells (ECs) of the dorsal aorta (DA), via endothelial-to-hematopoietic transition (EHT). The zebrafish is a primary model organism to study the process in vivo. While the role of mechanical stress in controlling gene expression promoting cell differentiation is actively investigated, mechanisms driving shape changes of the DA and individual ECs remain poorly understood. We address this problem by developing a new DA micromechanical model and applying it to experimental data on zebrafish morphogenesis. The model considers the DA as an isotropic tubular membrane subjected to hydrostatic blood pressure and axial stress. The DA evolution is described as a movement in the dimensionless controlling parameters space: normalized hydrostatic pressure and axial stress. We argue that HSPC production is accompanied by two mechanical instabilities arising in the system due to the plane stress in the DA walls and show how a complex interplay between mechanical forces in the system drives the emerging morphological changes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping