PUBLICATION
Synergistic effect of durian fruit rind polysaccharide gel encapsulated prebiotic and probiotic dietary supplements on growth performance, immune-related gene expression, and disease resistance in Zebrafish (Danio rerio)
- Authors
- Snega Priya, P., Ashwitha, A., Thamizharasan, K., Harishkumar, M., Dinesh, S., Nithya, T.G., Kamaraj, M.
- ID
- ZDB-PUB-210424-6
- Date
- 2021
- Source
- Heliyon 7: e06669 (Journal)
- Registered Authors
- Keywords
- Artemia nauplii, Bacillus subtilis, Bioencapsulation, Danio rerio, Durian fruit, Immune gene expression, Polysaccharide gel
- MeSH Terms
- none
- PubMed
- 33889779 Full text @ Heliyon
Citation
Snega Priya, P., Ashwitha, A., Thamizharasan, K., Harishkumar, M., Dinesh, S., Nithya, T.G., Kamaraj, M. (2021) Synergistic effect of durian fruit rind polysaccharide gel encapsulated prebiotic and probiotic dietary supplements on growth performance, immune-related gene expression, and disease resistance in Zebrafish (Danio rerio). Heliyon. 7:e06669.
Abstract
The present study investigated the effect of polysaccharide gel (PG) extracted from the rind of durian fruit which is encapsulated with Bacillus subtilis as a feed and co-inoculation with Artemia nauplii in the induction of immune response in Danio rerio after Vibrio immersion challenge (5 days). The total RBC count, lysozyme activity test, weight, and length analysis revealed that the zebra fishes fed with the PG encapsulated probiotics had more immune induction activity, survival, and growth than the non-encapsulated groups. When the expression of the immune-related genes was measured, the studies revealed the significant upregulation (P < 0 .05) of interleukin 1 beta (Il1b), lysozyme (lyz), tumor necrosis factor-alpha (TNF-α), superoxide dismutase (SOD) genes in fish fed with PG encapsulated probiotics with A. nauplii showed an immense effect on the induction of immune response compared to other feeds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping