PUBLICATION
Partial Hepatectomy in Adult Zebrafish
- Authors
- Oderberg, I.M., Goessling, W.
- ID
- ZDB-PUB-210420-12
- Date
- 2021
- Source
- Journal of visualized experiments : JoVE (170): (Journal)
- Registered Authors
- Goessling, Wolfram
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Hepatectomy*
- Liver/surgery*
- Liver Regeneration*
- Male
- Models, Animal*
- Zebrafish*
- PubMed
- 33871461 Full text @ J. Vis. Exp.
Citation
Oderberg, I.M., Goessling, W. (2021) Partial Hepatectomy in Adult Zebrafish. Journal of visualized experiments : JoVE. (170):.
Abstract
Liver failure is one of the leading causes of death worldwide, and mortality from chronic liver disease is rising sharply in the United States. Healthy livers are capable of regenerating from toxic damage, but in advanced liver disease, the natural ability of the liver to regenerate is impaired. Zebrafish have emerged as a powerful experimental system for studying regeneration. They are an ideal model for studying liver regeneration from partial hepatectomy, a procedure with direct clinical relevance in which part of the liver is surgically removed, leaving the rest intact. There is no standard protocol for partial hepatectomy; previous studies using this model have used slightly different protocols and reported disparate results. Described here is an efficient, reproducible protocol for performing a partial hepatectomy in adult zebrafish. We use this technique to demonstrate that zebrafish are capable of epimorphic regeneration of the resected lobe. This protocol can be used to further interrogate the mechanisms required for liver regeneration in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping