In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope
- Qin, X., Chen, C., Wang, L., Chen, X., Liang, Y., Jin, X., Pan, W., Liu, Z., Li, H., Yang, G.
- Biochemical and Biophysical Research Communications 557: 8-13 (Journal)
- Registered Authors
- Pan, Weijun
- Light-sheet illumination microscope, Maximum intensity projection, Virtual reality, Zebrafish intersegmental vessel
- MeSH Terms
- Animals, Genetically Modified
- Blood Vessels/embryology*
- Blood Vessels/growth & development
- Embryo, Nonmammalian/blood supply*
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Imaging, Three-Dimensional/methods
- Neovascularization, Physiologic
- Zebrafish/growth & development
- 33857842 Full text @ Biochem. Biophys. Res. Commun.
Qin, X., Chen, C., Wang, L., Chen, X., Liang, Y., Jin, X., Pan, W., Liu, Z., Li, H., Yang, G. (2021) In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope. Biochemical and Biophysical Research Communications. 557:8-13.
Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes