PUBLICATION

Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling

Authors
Brunt, L., Greicius, G., Rogers, S., Evans, B.D., Virshup, D.M., Wedgwood, K.C.A., Scholpp, S.
ID
ZDB-PUB-210409-3
Date
2021
Source
Nature communications   12: 2058 (Journal)
Registered Authors
Scholpp, Steffen
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning
  • Embryo, Nonmammalian/metabolism
  • Enzyme Activation
  • Fibroblasts/metabolism
  • Gastrulation
  • HEK293 Cells
  • Humans
  • JNK Mitogen-Activated Protein Kinases/metabolism
  • Membrane Proteins/metabolism*
  • Mice, Inbred C57BL
  • Neural Plate/embryology
  • Neural Plate/metabolism
  • Neurogenesis
  • Paracrine Communication
  • Pseudopodia/metabolism*
  • Systems Analysis
  • Telocytes/metabolism
  • Wnt Signaling Pathway*
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed
33824332 Full text @ Nat. Commun.
Abstract
Wnt signaling regulates cell proliferation and cell differentiation as well as migration and polarity during development. However, it is still unclear how the Wnt ligand distribution is precisely controlled to fulfil these functions. Here, we show that the planar cell polarity protein Vangl2 regulates the distribution of Wnt by cytonemes. In zebrafish epiblast cells, mouse intestinal telocytes and human gastric cancer cells, Vangl2 activation generates extremely long cytonemes, which branch and deliver Wnt protein to multiple cells. The Vangl2-activated cytonemes increase Wnt/β-catenin signaling in the surrounding cells. Concordantly, Vangl2 inhibition causes fewer and shorter cytonemes to be formed and reduces paracrine Wnt/β-catenin signaling. A mathematical model simulating these Vangl2 functions on cytonemes in zebrafish gastrulation predicts a shift of the signaling gradient, altered tissue patterning, and a loss of tissue domain sharpness. We confirmed these predictions during anteroposterior patterning in the zebrafish neural plate. In summary, we demonstrate that Vangl2 is fundamental to paracrine Wnt/β-catenin signaling by controlling cytoneme behaviour.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes