|ZFIN ID: ZDB-PUB-210326-9|
In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration
Pronobis, M.I., Zheng, S., Singh, S.P., Goldman, J.A., Poss, K.D.
|Source:||eLIFE 10: (Journal)|
|Registered Authors:||Goldman, Joseph, Poss, Kenneth D., Pronobis, Mira, Singh, Sumeet Pal|
|Keywords:||developmental biology, zebrafish|
|PubMed:||33764296 Full text @ Elife|
Pronobis, M.I., Zheng, S., Singh, S.P., Goldman, J.A., Poss, K.D. (2021) In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. eLIFE. 10:.
ABSTRACTStrategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa or Vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.
ADDITIONAL INFORMATION No data available