PUBLICATION
Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish
- Authors
- Zhang, F., Zhang, X., Gu, Y., Wang, M., Guo, S., Liu, J., Zhang, X., Zhao, Z., Qian, B., Yan, Y., Yu, L., Xu, C., Liu, C., Cao, F., Qian, D., Duan, J.A.
- ID
- ZDB-PUB-210309-8
- Date
- 2021
- Source
- Oxidative medicine and cellular longevity 2021: 3923625 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Apoptosis/genetics
- Cell Cycle/drug effects
- Cell Cycle/genetics
- Cell Line
- Disease Models, Animal
- Gene Expression Regulation/drug effects
- Hepatocytes/drug effects
- Hepatocytes/metabolism
- Hepatocytes/pathology*
- Humans
- Hydrogen Peroxide/toxicity
- Larva/drug effects
- Larva/genetics
- Larva/physiology
- Liver/pathology*
- Lycium/chemistry*
- Non-alcoholic Fatty Liver Disease/genetics
- Non-alcoholic Fatty Liver Disease/pathology
- Oxidative Stress/drug effects*
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology*
- Protective Agents/pharmacology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Spectroscopy, Fourier Transform Infrared
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 33680282 Full text @ Oxid Med Cell Longev
Citation
Zhang, F., Zhang, X., Gu, Y., Wang, M., Guo, S., Liu, J., Zhang, X., Zhao, Z., Qian, B., Yan, Y., Yu, L., Xu, C., Liu, C., Cao, F., Qian, D., Duan, J.A. (2021) Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval Zebrafish. Oxidative medicine and cellular longevity. 2021:3923625.
Abstract
Scavenging of oxidative stress by antioxidants may provide a therapeutic strategy for nonalcoholic fatty liver disease (NAFLD). Increasing evidence is supporting the potential application of natural resourced polysaccharides as promising prevention or treatment strategies against NAFLD. In the current study, an acidic heteropolysaccharide, LFP-a1, was isolated and purified from Lycii fructus with successively hot water refluxing extraction, alcohol precipitation, protein removal, and DEAE-52 cellulose chromatographic separation. LFP-a1 was a complicated structured polysaccharide with an average MW of 4.74 × 104 Da and composed of 6 monosaccharides and 1 uronic acid. Preexposure of LFP-a1 could increase the cell viability and reverse the abnormal oxidative stress though inhibition of mitochondrial-mediated apoptotic pathway and correction of cell cycle progression against H2O2 hepatoxicity in NAFLD model L02 cells. Consistently, in vivo study in thioacetamide- (TAA-) induced NAFLD model zebrafish larvae showed LFP-a1 preserved the liver integrity and alleviated TAA-induced oxidative stress through downregulation of abnormal apoptosis. These observations indicated the hepatoprotective activity of LFP-a1, which may be applied for the prevention or treatment of NAFLD or other oxidative stress-related diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping