PUBLICATION
Agaricus bisporus-Derived Glucosamine Hydrochloride Facilitates Skeletal Injury Repair through Bmp Signaling in Zebrafish Osteoporosis Model
- Authors
- Peng, W., Zhang, W., Wu, Q., Cai, S., Jia, T., Sun, J., Lin, Z., Alitongbieke, G., Chen, Y., Su, Y., Lin, J., Cai, L., Sun, Y., Pan, Y., Xue, Y.
- ID
- ZDB-PUB-210227-9
- Date
- 2021
- Source
- Journal of natural products 84(4): 1294-1305 (Journal)
- Registered Authors
- Xue, Yu
- Keywords
- none
- MeSH Terms
-
- Agaricus/chemistry*
- Animals
- Bone Morphogenetic Proteins/metabolism*
- Bone and Bones/drug effects*
- Disease Models, Animal
- Glucosamine/pharmacology*
- Larva/drug effects
- Osteoporosis/drug therapy*
- Regeneration
- Signal Transduction/drug effects*
- Skeleton/drug effects
- Zebrafish
- PubMed
- 33635072 Full text @ J. Nat. Prod.
Citation
Peng, W., Zhang, W., Wu, Q., Cai, S., Jia, T., Sun, J., Lin, Z., Alitongbieke, G., Chen, Y., Su, Y., Lin, J., Cai, L., Sun, Y., Pan, Y., Xue, Y. (2021) Agaricus bisporus-Derived Glucosamine Hydrochloride Facilitates Skeletal Injury Repair through Bmp Signaling in Zebrafish Osteoporosis Model. Journal of natural products. 84(4):1294-1305.
Abstract
Glucosamine hydrochloride (GAH), one of the most basic and important derivatives of chitin, is obtained by hydrolysis of chitin in concentrated hydrochloric acid. At present, little is known about how GAH functions in skeletal development. In this report, we demonstrate that GAH, extracted from the cell wall of Agaricus bisporus, acts in a dose-dependent manner to promote not only cartilage and bone development in larvae but also caudal fin regeneration in adult fish. Furthermore, GAH treatment causes a significant increase in expression of bone-related marker genes, indicating its important role in promoting skeletal development. We show that in both larval and adult osteoporosis models induced by high iron osteogenic defects are significantly ameliorated after treatment with GAH, which regulates expression of a series of bone-related genes. Finally, we demonstrate that GAH promotes skeletal development and injury repair through bone morphogenetic protein (Bmp) signaling, and it works at the downstream of the receptor level. Taken together, our findings not only provide a strong research foundation and strategy for the screening of natural osteoporosis drugs and product development using a zebrafish model but also establish the potential for the development of Agaricus bisporus-derived GAH as a new drug for osteoporosis treatment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping