PUBLICATION
Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining
- Authors
- Rostam, N., Dosch, R.
- ID
- ZDB-PUB-210220-39
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 245-252 (Chapter)
- Registered Authors
- Dosch, Roland
- Keywords
- Bucky ball, Fixation method, Germ plasm, Glyoxal, Immunostaining, PFA, Zebrafish
- MeSH Terms
-
- Female
- Fixatives/metabolism
- Zebrafish Proteins/metabolism
- Tissue Fixation/methods*
- Male
- Embryo, Mammalian/metabolism*
- Glyoxal/metabolism*
- Animals
- Antibodies/metabolism
- Immunohistochemistry/methods*
- Staining and Labeling/methods
- Zebrafish/metabolism*
- PubMed
- 33606236 Full text @ Meth. Mol. Biol.
Citation
Rostam, N., Dosch, R. (2021) Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining. Methods in molecular biology (Clifton, N.J.). 2218:245-252.
Abstract
Immunohistochemistry has been widely used as a robust technique to determine the cellular and subcellular localization of proteins. This information ultimately helps to understand the function of these proteins and how biological processes are regulated. Antibodies applicable for labeling in zebrafish are limited, making immuno-staining challenging. Recently glyoxal fixation was rediscovered in tissue culture, mouse, rat, and Drosophila, expanding the list of effective antibodies for these species. Here, we compare a protocol for zebrafish staining using glyoxal as a fixative agent with PFA. We demonstrate that glyoxal fixation improves the antigenicity of some epitopes thereby increasing the number of useful antibodies in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping