PUBLICATION
Quantifying Tissue Tension in the Granulosa Layer After Laser Surgery
- Authors
- Xia, P., Heisenberg, C.P.
- ID
- ZDB-PUB-210220-31
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 117-128 (Chapter)
- Registered Authors
- Heisenberg, Carl-Philipp
- Keywords
- Granulosa cells, Laser ablation, Morphogenesis, Tissue tension, Zebrafish folliculogenesis
- MeSH Terms
-
- Animals
- Female
- Granulosa Cells/cytology*
- Laser Therapy/methods*
- Morphogenesis/physiology
- Zebrafish/physiology
- PubMed
- 33606227 Full text @ Meth. Mol. Biol.
Citation
Xia, P., Heisenberg, C.P. (2021) Quantifying Tissue Tension in the Granulosa Layer After Laser Surgery. Methods in molecular biology (Clifton, N.J.). 2218:117-128.
Abstract
Tissue morphogenesis is driven by mechanical forces triggering cell movements and shape changes. Quantitatively measuring tension within tissues is of great importance for understanding the role of mechanical signals acting on the cell and tissue level during morphogenesis. Here we introduce laser ablation as a useful tool to probe tissue tension within the granulosa layer, an epithelial monolayer of somatic cells that surround the zebrafish female gamete during folliculogenesis. We describe in detail how to isolate follicles, mount samples, perform laser surgery, and analyze the data.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping