PUBLICATION
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid
- Authors
- Marqueño, A., Flores, C., Casado, M., Porte, C.
- ID
- ZDB-PUB-210211-1
- Date
- 2021
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 231: 105733 (Journal)
- Registered Authors
- Keywords
- Fish cells, Gene expression, Lipid metabolism, Liver, Triglycerides
- MeSH Terms
-
- Animals
- Cell Line
- Cell Survival/drug effects
- Cyprinidae
- Gene Expression Regulation/drug effects
- Hepatocytes/drug effects
- Hepatocytes/metabolism
- Lipid Metabolism*/drug effects
- Lipids/analysis
- Peroxisome Proliferator-Activated Receptors/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Retinoid X Receptors/metabolism
- Tretinoin/toxicity*
- Trialkyltin Compounds/toxicity*
- Water Pollutants, Chemical/toxicity
- Zebrafish
- PubMed
- 33429301 Full text @ Aquat. Toxicol.
Citation
Marqueño, A., Flores, C., Casado, M., Porte, C. (2021) Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid. Aquatic toxicology (Amsterdam, Netherlands). 231:105733.
Abstract
There is increasing awareness that exposure to endocrine disrupters interferes with lipid homeostasis in vertebrates, including fish. Many of these compounds exert their action by binding to nuclear receptors, such as peroxisome proliferator-activated receptors and retinoid X receptor. This work investigates the use of fish liver cells (PLHC-1 and ZFL cells) for the screening of metabolic and lipid disrupters in the aquatic environment by assessing changes in the cell's lipidome after exposure to the model compounds, tributyltin chloride and all-trans retinoic acid. Lipid extracts, analyzed by FIA-ESI (+/-) Orbitrap, evidenced the intracellular accumulation of triglycerides and diglycerides in both cell models after exposure to 100 and 200 nM tributyltin chloride for 24 h. Exposure to 1 μM all-trans retinoic acid led to a significant accumulation of triglycerides in PLHC-1 cells, while few triglycerides were accumulated in ZFL cells. Retinoic acid (cyp26b1, cyp3a65, lrata) and lipid metabolism (fasn, scd, elovl6) related genes were up-regulated by tributyltin chloride and all-trans retinoic acid, while only all-trans retinoic acid down-regulated the expression of dgat1a. The two cell models show sensitivity and responses to tributyltin chloride and all-trans retinoic acid comparable to those previously reported in mammalian cells. These results support the use of fish liver cells as alternative models for the detection of contaminants that act as lipid disrupters in the aquatic environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping