PUBLICATION
Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation
- Authors
- Aljiboury, A.A., Mujcic, A., Cammerino, T., Rathbun, L.I., Hehnly, H.
- ID
- ZDB-PUB-210209-12
- Date
- 2021
- Source
- STAR protocols 2: 100293 (Other)
- Registered Authors
- Keywords
- Cell biology, Microscopy, Model organisms
- MeSH Terms
-
- Animals
- Centrosome/metabolism*
- Embryo, Nonmammalian/embryology*
- Microscopy, Confocal
- Mitosis/drug effects*
- Protein Kinase Inhibitors/pharmacology*
- Spindle Apparatus/metabolism*
- Zebrafish/embryology*
- PubMed
- 33554134 Full text @ STAR Protoc
Citation
Aljiboury, A.A., Mujcic, A., Cammerino, T., Rathbun, L.I., Hehnly, H. (2021) Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation. STAR protocols. 2:100293.
Abstract
During the earliest division stages, zebrafish embryos have large cells that divide rapidly and synchronously to create a cellular layer on top of the yolk. Here, we describe a protocol for monitoring spindle dynamics during these early embryonic divisions. We outline techniques for injecting zebrafish embryos with small-molecule inhibitors toward polo-like kinases, preparing and mounting embryos for three-dimensional imaging using confocal microscopy. These techniques are used to understand how the early zebrafish embryo's centrosome constructs the mitotic spindle. For complete details on the use and execution of this protocol, please refer to Rathbun et al. (2020).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping