PUBLICATION
Development of curcumin-loaded zein nanoparticles for transport across the blood-brain barrier and inhibition of glioblastoma cell growth
- Authors
- Zhang, H., van Os, W.L., Tian, X., Zu, G., Ribovski, L., Bron, R., Bussmann, J., Kros, A., Liu, Y., Zuhorn, I.S.
- ID
- ZDB-PUB-210205-15
- Date
- 2021
- Source
- Biomaterials science 9(21): 7092-7103 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood-Brain Barrier
- Cell Line, Tumor
- Cell Proliferation
- Curcumin*/pharmacology
- Curcumin*/therapeutic use
- Drug Delivery Systems
- Glioblastoma*/drug therapy
- Nanoparticles*
- Zebrafish
- Zein*
- PubMed
- 33538729 Full text @ Biomater Sci
Citation
Zhang, H., van Os, W.L., Tian, X., Zu, G., Ribovski, L., Bron, R., Bussmann, J., Kros, A., Liu, Y., Zuhorn, I.S. (2021) Development of curcumin-loaded zein nanoparticles for transport across the blood-brain barrier and inhibition of glioblastoma cell growth. Biomaterials science. 9(21):7092-7103.
Abstract
Glioblastoma (GBM) is a devastating primary brain tumor resistant to conventional therapies. A major obstacle to GBM treatment is the blood-brain barrier (BBB), or blood-glioma barrier, which prevents the transport of systemically administered (chemotherapeutic) drugs into the tumor. This study reports the design of dodecamer peptide (G23)-functionalized polydopamine (pD)-coated curcumin-loaded zein nanoparticles (CUR-ZpD-G23 NPs) that efficiently traversed the BBB, and delivered curcumin to glioblastoma cells. The NPs enhanced the cellular uptake of curcumin by C6 glioma cells compared to free curcumin, and showed high penetration into 3D tumor spheroids. Functionalization of the NPs with G23 stimulated BBB crossing and tumor spheroid penetration. Moreover, the NPs markedly inhibited proliferation and migration and induced cell death in liquid and soft agar models of C6 glioma cell growth. Fluorescence microscopy and flow cytometry studies showed that the CUR-ZpD-G23 NPs increased cellular ROS production and induced apoptosis of C6 glioma cells. Following in vivo intravenous injection in zebrafish, ZpD-G23 NPs demonstrated the ability to circulate, which is a first prerequisite for their use in targeted drug delivery. In conclusion, zein-polydopamine-G23 NPs show potential as a drug delivery platform for therapy of GBM, which requires further validation in in vivo glioblastoma models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping