PUBLICATION
Examining Muscle Regeneration in Zebrafish Models of Muscle Disease
- Authors
- Montandon, M., Currie, P.D., Ruparelia, A.A.
- ID
- ZDB-PUB-210202-19
- Date
- 2021
- Source
- Journal of visualized experiments : JoVE (167): (Journal)
- Registered Authors
- Currie, Peter D., Montandon, Margo
- Keywords
- none
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Embryo, Nonmammalian/physiopathology
- Genotype
- Laminin/deficiency
- Laminin/metabolism
- Larva/physiology
- Muscle, Skeletal/diagnostic imaging
- Muscle, Skeletal/pathology
- Muscle, Skeletal/physiopathology*
- Muscular Diseases/pathology
- Muscular Diseases/physiopathology*
- Regeneration/physiology*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 33522516 Full text @ J. Vis. Exp.
Citation
Montandon, M., Currie, P.D., Ruparelia, A.A. (2021) Examining Muscle Regeneration in Zebrafish Models of Muscle Disease. Journal of visualized experiments : JoVE. (167):.
Abstract
Skeletal muscle has a remarkable ability to regenerate following injury, which is driven by obligate tissue resident muscle stem cells. Following injury, the muscle stem cell is activated and undergoes cell proliferation to generate a pool of myoblasts, which subsequently differentiate to form new muscle fibers. In many muscle wasting conditions, including muscular dystrophy and ageing, this process is impaired resulting in the inability of muscle to regenerate. The process of muscle regeneration in zebrafish is highly conserved with mammalian systems providing an excellent system to study muscle stem cell function and regeneration, in muscle wasting conditions such as muscular dystrophy. Here, we present a method to examine muscle regeneration in zebrafish models of muscle disease. The first step involves the use of a genotyping platform that allows the determination of the genotype of the larvae prior to eliciting an injury. Having determined the genotype, the muscle is injured using a needle stab, following which polarizing light microscopy is used to determine the extent of muscle regeneration. We therefore provide a high throughput pipeline which allows the examination of muscle regeneration in zebrafish models of muscle disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping