PUBLICATION
Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis
- Authors
- Clark, E.M., Link, B.A.
- ID
- ZDB-PUB-210114-16
- Date
- 2021
- Source
- Molecular biology of the cell 32(5): 391-401 (Journal)
- Registered Authors
- Link, Brian
- Keywords
- none
- MeSH Terms
-
- Animals
- Antigens, Neoplasm/genetics
- Antigens, Neoplasm/metabolism
- Aryl Hydrocarbon Receptor Nuclear Translocator/genetics*
- Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism
- COP-Coated Vesicles/metabolism
- Carrier Proteins/metabolism
- Endoplasmic Reticulum/metabolism
- Golgi Apparatus/metabolism
- Homeostasis
- Neoplasm Proteins/genetics*
- Neoplasm Proteins/metabolism
- Protein Transport/physiology
- Vesicular Transport Proteins/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 33439675 Full text @ Mol. Biol. Cell
Citation
Clark, E.M., Link, B.A. (2021) Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis. Molecular biology of the cell. 32(5):391-401.
Abstract
Coat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their potential distinct and complementary functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue specific defects in the building of heart, muscle, lens, and intestine, in addition to the previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping