PUBLICATION

Control of osteoblast regeneration by a train of Erk activity waves

Authors
De Simone, A., Evanitsky, M.N., Hayden, L., Cox, B.D., Wang, J., Tornini, V.A., Ou, J., Chao, A., Poss, K.D., Di Talia, S.
ID
ZDB-PUB-210109-13
Date
2021
Source
Nature   590(7844): 129-133 (Journal)
Registered Authors
Cox, Ben, Ou, Jianhong, Poss, Kenneth D., Tornini, Valerie A.
Keywords
none
Datasets
GEO:GSE147551
MeSH Terms
  • Animal Scales/cytology
  • Animal Scales/enzymology
  • Animal Scales/growth & development
  • Animal Scales/physiology
  • Animals
  • Diffusion
  • Extracellular Signal-Regulated MAP Kinases/metabolism*
  • Female
  • MAP Kinase Signaling System*
  • Male
  • Osteoblasts/cytology*
  • Osteoblasts/metabolism*
  • Regeneration*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed
33408418 Full text @ Nature
Abstract
Regeneration is a complex chain of events that restores a tissue to its original size and shape. The tissue-wide coordination of cellular dynamics that is needed for proper morphogenesis is challenged by the large dimensions of regenerating body parts. Feedback mechanisms in biochemical pathways can provide effective communication across great distances1-5, but how they might regulate growth during tissue regeneration is unresolved6,7. Here we report that rhythmic travelling waves of Erk activity control the growth of bone in time and space in regenerating zebrafish scales, millimetre-sized discs of protective body armour. We find that waves of Erk activity travel across the osteoblast population as expanding concentric rings that are broadcast from a central source, inducing ring-like patterns of tissue growth. Using a combination of theoretical and experimental analyses, we show that Erk activity propagates as excitable trigger waves that are able to traverse the entire scale in approximately two days and that the frequency of wave generation controls the rate of scale regeneration. Furthermore, the periodic induction of synchronous, tissue-wide activation of Erk in place of travelling waves impairs tissue growth, which indicates that wave-distributed Erk activation is key to regeneration. Our findings reveal trigger waves as a regulatory strategy to coordinate cell behaviour and instruct tissue form during regeneration.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes