PUBLICATION
A new reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development
- Authors
- Frétaud, M., Do Khoa, N., Houel, A., Lunazzi, A., Boudinot, P., Langevin, C.
- ID
- ZDB-PUB-201229-44
- Date
- 2020
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 250(5): 701-716 (Journal)
- Registered Authors
- Langevin, Christelle
- Keywords
- 3D imaging, Lymphatic network, infectiology, tissue clearing, zebrafish phenotyping
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Endothelial Cells*
- Genes, Reporter*
- Lymphatic Vessels/cytology*
- Zebrafish*
- PubMed
- 33369805 Full text @ Dev. Dyn.
Citation
Frétaud, M., Do Khoa, N., Houel, A., Lunazzi, A., Boudinot, P., Langevin, C. (2020) A new reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(5):701-716.
Abstract
Background In zebrafish, lymphatic endothelial cells (LECs) originate from multiple/several distinct progenitor populations and generate organ-specific lymphatic vasculatures. Cell fate and tissue specificities were determined using a combination of genetically engineered transgenic lines in which the promoter of a LEC-specific gene drives expression of a fluorescent reporter protein.
Results We established a novel zebrafish transgenic line expressing eGFP under the control of part of the zebrafish batf3 promoter (Basic Leucine Zipper ATF-Like Transcription Factor 3). Spatiotemporal examination of Tg(batf3MIN:eGFP) transgenic fish revealed a typical lymphatic expression pattern, which does not perfectly recapitulate the expression pattern of existing LEC transgenic lines. eGFP+ cells constitute a heterogeneous endothelial cell population, which expressed LEC and/or blood endothelial cells (BEC) markers in different tissues. In addition, we characterize the renal eGFP+ cell as a population of interest to study kidney diseases and regeneration.
Conclusion Our Tg(batf3MIN:eGFP) reporter zebrafish line provides a useful system to study LEC populations, of which heterogeneity depends on origin of progenitors, tissue environment and physiological conditions. We further developed a novel fish-adapted tissue clearing method, which allows deep imaging and 3D-visualization of vascular and lymphatic networks in the whole organism. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping