PUBLICATION
Visualizing the metazoan proliferation-quiescence decision in vivo
- Authors
- Adikes, R.C., Kohrman, A.Q., Martinez, M.A.Q., Palmisano, N.J., Smith, J.J., Medwig-Kinney, T.N., Min, M., Sallee, M.D., Ahmed, O.B., Kim, N., Liu, S., Morabito, R.D., Weeks, N., Zhao, Q., Zhang, W., Feldman, J.L., Barkoulas, M., Pani, A.M., Spencer, S.L., Martin, B.L., Matus, D.Q.
- ID
- ZDB-PUB-201223-3
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Martin, Benjamin
- Keywords
- C. elegans, cell biology, developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Biosensing Techniques/methods*
- Caenorhabditis elegans/cytology*
- Caenorhabditis elegans Proteins/metabolism
- Cell Cycle/physiology
- Cell Division
- Cell Proliferation/physiology
- Cyclin-Dependent Kinases/metabolism
- PubMed
- 33350383 Full text @ Elife
Citation
Adikes, R.C., Kohrman, A.Q., Martinez, M.A.Q., Palmisano, N.J., Smith, J.J., Medwig-Kinney, T.N., Min, M., Sallee, M.D., Ahmed, O.B., Kim, N., Liu, S., Morabito, R.D., Weeks, N., Zhao, Q., Zhang, W., Feldman, J.L., Barkoulas, M., Pani, A.M., Spencer, S.L., Martin, B.L., Matus, D.Q. (2020) Visualizing the metazoan proliferation-quiescence decision in vivo. eLIFE. 9:.
Abstract
Cell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in C. elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from quiescent cells in G0, revealing a possible commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. Finally, we derive a predictive model of future proliferation behavior in C. elegans based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell cycle control in a wide-range of developmental contexts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping