PUBLICATION
Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays
- Authors
- Nakayama, H., Hata, K., Matsuoka, I., Zang, L., Kim, Y., Chu, D., Juneja, L.R., Nishimura, N., Shimada, Y.
- ID
- ZDB-PUB-201217-2
- Date
- 2020
- Source
- Molecules 25(24): (Journal)
- Registered Authors
- Keywords
- diabetes, drug discovery, metabolic syndrome, screening
- MeSH Terms
-
- 3T3-L1 Cells
- Adipocytes/drug effects
- Adipogenesis/drug effects*
- Animals
- Anti-Obesity Agents/chemistry*
- Anti-Obesity Agents/pharmacology*
- Biological Products/chemistry*
- Biological Products/pharmacology*
- Cells, Cultured
- Drug Evaluation, Preclinical/methods
- Mice
- Zebrafish
- PubMed
- 33322023 Full text @ Molecules
Citation
Nakayama, H., Hata, K., Matsuoka, I., Zang, L., Kim, Y., Chu, D., Juneja, L.R., Nishimura, N., Shimada, Y. (2020) Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays. Molecules. 25(24):.
Abstract
(1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping