PUBLICATION
Polystyrene nanoparticles trigger the activation of p38 MAPK and apoptosis via inducing oxidative stress in zebrafish and macrophage cells
- Authors
- Hu, Q., Wang, H., He, C., Jin, Y., Fu, Z.
- ID
- ZDB-PUB-201215-15
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 269: 116075 (Journal)
- Registered Authors
- Keywords
- Oxidative stress, Polystyrene nanoparticles, Transcriptomic analysis, Zebrafish, p38 MAPK pathway
- MeSH Terms
-
- Animals
- Apoptosis
- Macrophages/metabolism
- Nanoparticles*/toxicity
- Oxidative Stress
- Polystyrenes*/metabolism
- Zebrafish/metabolism
- p38 Mitogen-Activated Protein Kinases/metabolism
- PubMed
- 33316494 Full text @ Environ. Pollut.
Citation
Hu, Q., Wang, H., He, C., Jin, Y., Fu, Z. (2020) Polystyrene nanoparticles trigger the activation of p38 MAPK and apoptosis via inducing oxidative stress in zebrafish and macrophage cells. Environmental pollution (Barking, Essex : 1987). 269:116075.
Abstract
Polystyrene nanoparticles (PS NPs), originated from breakdown of large plastic wastes, have already caused much concern for their environmental risks on health. This current study was aimed to reveal the toxicological mechanism of PS NPs on developing zebrafish and macrophage cells. To fulfill this purpose, 42 nm PS NPs were exposed to the early development stage of zebrafish for 5 days, the decreased heart rate and locomotor activity of zebrafish larvae were observed. The fluorescent PS NPs were used to precisely assess the accumulation of PS NPs in zebrafish larvae, and the results indicated that PS NPs not only accumulated in digestive system, but also infiltrated into the liver. More importantly, the transcriptomic analysis revealed that a total of 356 genes were differentially expressed and the KEGG class map showed significant differences in the MAPK pathway upon PS NPs treatment. Meanwhile, the induction of oxidative stress and inflammation were also observed in zebrafish larvae. Furthermore, PS NPs also induced oxidative damage and inflammatory response in RAW 264.7 cells, which activated p38 MAPK signal pathway and finally induced cell apoptosis. Our study provides a new understanding of MAPK signaling pathway involved in toxicity mechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping