PUBLICATION
foxl2 and foxl3 are two ancient paralogs that remain fully functional in teleosts
- Authors
- Crespo, B., Lan-Chow-Wing, O., Rocha, A., Zanuy, S., Gómez, A.
- ID
- ZDB-PUB-201210-13
- Date
- 2013
- Source
- General and comparative endocrinology 194: 81-93 (Journal)
- Registered Authors
- Keywords
- Foxl2, Genome duplication, Ovarian development, Reproduction, Transcription factor
- MeSH Terms
-
- 3' Untranslated Regions/genetics
- 5' Untranslated Regions/genetics
- Amino Acid Sequence
- Animals
- Bass/genetics*
- Bass/metabolism*
- DNA, Complementary/genetics
- Female
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism*
- Male
- Molecular Sequence Data
- Real-Time Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- PubMed
- 24045113 Full text @ Gen. Comp. Endocrinol.
Citation
Crespo, B., Lan-Chow-Wing, O., Rocha, A., Zanuy, S., Gómez, A. (2013) foxl2 and foxl3 are two ancient paralogs that remain fully functional in teleosts. General and comparative endocrinology. 194:81-93.
Abstract
FOXL2 is a forkhead transcription factor involved in mammalian development and regulation of reproduction. Two foxl2 paralogs, foxl2a and foxl2b, have been described in various teleost species and were considered as fish-specific duplicates. Here, we report the isolation and characterization of foxl2a (foxl2) and foxl2b (foxl3) in European sea bass (Dicentrarchus labrax), together with the identification of these two genes in non-teleost genomes. Phylogenetic and synteny analyses indicate that these paralogs originated from an ancient genome duplication event that happened long before the teleost specific duplication. While foxl2/foxl2a has been maintained in most vertebrate lineages, foxl2b, which we propose to rename as foxl3, was repeatedly lost in tetrapods. Gonadal expression patterns of the sea bass genes point to a strong sexual dimorphism, and the mRNA levels of foxl2 in ovary and foxl3 in testis vary significantly during the reproductive cycle. When overexpressed in cultured ovarian follicular cells, foxl2 and foxl3 produced functional transcription factors able to control the expression of reproduction-related genes. Taken together, these data suggest that Foxl2 may play a conserved role in ovarian maturation, while Foxl3 could be involved in testis physiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping