PUBLICATION

Histone H3.3 beyond cancer: Germline mutations in Histone 3 Family 3A and 3B cause a previously unidentified neurodegenerative disorder in 46 patients

Authors
Bryant, L., Li, D., Cox, S.G., Marchione, D., Joiner, E.F., Wilson, K., Janssen, K., Lee, P., March, M.E., Nair, D., Sherr, E., Fregeau, B., Wierenga, K.J., Wadley, A., Mancini, G.M.S., Powell-Hamilton, N., van de Kamp, J., Grebe, T., Dean, J., Ross, A., Crawford, H.P., Powis, Z., Cho, M.T., Willing, M.C., Manwaring, L., Schot, R., Nava, C., Afenjar, A., Lessel, D., Wagner, M., Klopstock, T., Winkelmann, J., Catarino, C.B., Retterer, K., Schuette, J.L., Innis, J.W., Pizzino, A., Lüttgen, S., Denecke, J., Strom, T.M., Monaghan, K.G., DDD Study, Yuan, Z.F., Dubbs, H., Bend, R., Lee, J.A., Lyons, M.J., Hoefele, J., Günthner, R., Reutter, H., Keren, B., Radtke, K., Sherbini, O., Mrokse, C., Helbig, K.L., Odent, S., Cogne, B., Mercier, S., Bezieau, S., Besnard, T., Kury, S., Redon, R., Reinson, K., Wojcik, M.H., Õunap, K., Ilves, P., Innes, A.M., Kernohan, K.D., Care4Rare Canada Consortium, Costain, G., Meyn, M.S., Chitayat, D., Zackai, E., Lehman, A., Kitson, H., CAUSES Study, Martin, M.G., Martinez-Agosto, J.A., Undiagnosed Diseases Network, Nelson, S.F., Palmer, C.G.S., Papp, J.C., Parker, N.H., Sinsheimer, J.S., Vilain, E., Wan, J., Yoon, A.J., Zheng, A., Brimble, E., Ferrero, G.B., Radio, F.C., Carli, D., Barresi, S., Brusco, A., Tartaglia, M., Thomas, J.M., Umana, L., Weiss, M.M., Gotway, G., Stuurman, K.E., Thompson, M.L., McWalter, K., Stumpel, C.T.R.M., Stevens, S.J.C., Stegmann, A.P.A., Tveten, K., Vøllo, A., Prescott, T., Fagerberg, C., Laulund, L.W., Larsen, M.J., Byler, M., Lebel, R.R., Hurst, A.C., Dean, J., Schrier Vergano, S.A., Norman, J., Mercimek-Andrews, S., Neira, J., Van Allen, M.I., Longo, N., Sellars, E., Louie, R.J., Cathey, S.S., Brokamp, E., Heron, D., Snyder, M., Vanderver, A., Simon, C., de la Cruz, X., Padilla, N., Crump, J.G., Chung, W., Garcia, B., Hakonarson, H.H., Bhoj, E.J.
ID
ZDB-PUB-201208-7
Date
2020
Source
Science advances   6(49): (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Forkhead Transcription Factors/genetics
  • Germ-Line Mutation
  • Histones*/genetics
  • Histones*/metabolism
  • Humans
  • Neurodegenerative Diseases*/genetics
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed
33268356 Full text @ Sci Adv
Abstract
Although somatic mutations in Histone 3.3 (H3.3) are well-studied drivers of oncogenesis, the role of germline mutations remains unreported. We analyze 46 patients bearing de novo germline mutations in histone 3 family 3A (H3F3A) or H3F3B with progressive neurologic dysfunction and congenital anomalies without malignancies. Molecular modeling of all 37 variants demonstrated clear disruptions in interactions with DNA, other histones, and histone chaperone proteins. Patient histone posttranslational modifications (PTMs) analysis revealed notably aberrant local PTM patterns distinct from the somatic lysine mutations that cause global PTM dysregulation. RNA sequencing on patient cells demonstrated up-regulated gene expression related to mitosis and cell division, and cellular assays confirmed an increased proliferative capacity. A zebrafish model showed craniofacial anomalies and a defect in Foxd3-derived glia. These data suggest that the mechanism of germline mutations are distinct from cancer-associated somatic histone mutations but may converge on control of cell proliferation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping