PUBLICATION

An Irf6-Esrp1/2 regulatory axis controls midface morphogenesis in vertebrates

Authors
Carroll, S.H., Macias Trevino, C., Li, E.B., Kawasaki, K., Myers, N., Hallett, S.A., Alhazmi, N., Cotney, J., Carstens, R.P., Liao, E.C.
ID
ZDB-PUB-201126-5
Date
2020
Source
Development (Cambridge, England)   147(24): (Journal)
Registered Authors
Liao, Eric
Keywords
Cleft, Craniofacial, Development, ESRP1, IRF6
Datasets
GEO:GSE153828
MeSH Terms
  • Animals
  • Ectoderm/growth & development
  • Ectoderm/metabolism
  • Epithelium/growth & development
  • Gene Expression Regulation, Developmental/genetics
  • Humans
  • Interferon Regulatory Factors/genetics*
  • Maxillofacial Development/genetics*
  • Mice
  • Morphogenesis/genetics*
  • Mutation/genetics
  • Neural Crest/growth & development
  • RNA-Binding Proteins/genetics*
  • SOXE Transcription Factors/genetics
  • Zebrafish
  • Zebrafish Proteins/genetics
PubMed
33234718 Full text @ Development
Abstract
Irf6 and Esrp1 are important for palate development across vertebrates. In zebrafish, we found that irf6 regulates the expression of esrp1 We detailed overlapping Irf6 and Esrp1/2 expression in mouse orofacial epithelium. In zebrafish, irf6 and esrp1/2 share expression in periderm, frontonasal ectoderm, and oral epithelium. Genetic disruption of irf6 and esrp1/2 in zebrafish resulted in cleft of the anterior neurocranium. The esrp1/2 mutant also developed cleft of the mouth opening. Lineage tracing of cranial neural crest cells revealed that cleft resulted not from migration defect, but from impaired chondrogenesis. Analysis of aberrant cells within the cleft revealed expression of sox10, col1a1 and irf6 and were adjacent to cells krt4 and krt5 positive. Breeding of mouse Irf6;Esrp1;Esrp2 compound mutants suggested genetic interaction, as the triple homozygote and the Irf6;Esrp1 double homozygote was not observed. Further, Irf6 heterozygosity reduced Esrp1/2 cleft severity. These studies highlight the complementary analysis of Irf6 and Esrp1/2 in mouse and zebrafish and captured a unique aberrant cell population expressing sox10, col1a1 and irf6 Future work characterizing this cell population will yield additional insight into cleft pathogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes