PUBLICATION
Developmental toxicity of the novel PFOS alternative OBS in developing zebrafish: An emphasis on cilia disruption
- Authors
- Huang, J., Sun, L., Mennigen, J.A., Liu, Y., Liu, S., Zhang, M., Wang, Q., Tu, W.
- ID
- ZDB-PUB-201124-2
- Date
- 2020
- Source
- Journal of hazardous materials 409: 124491 (Journal)
- Registered Authors
- Keywords
- Ciliary dysfunction, Developmental toxicity, OBS, PFOS alternative, Zebrafish
- MeSH Terms
-
- Alkanesulfonic Acids*/toxicity
- Animals
- Cilia
- Fluorocarbons*/toxicity
- Molecular Docking Simulation
- Water Pollutants, Chemical*
- Zebrafish
- PubMed
- 33223314 Full text @ J. Hazard. Mater.
Citation
Huang, J., Sun, L., Mennigen, J.A., Liu, Y., Liu, S., Zhang, M., Wang, Q., Tu, W. (2020) Developmental toxicity of the novel PFOS alternative OBS in developing zebrafish: An emphasis on cilia disruption. Journal of hazardous materials. 409:124491.
Abstract
In recent years, sodium p-perfluorous nonenoxybenzene sulfonate (OBS) has emerged as a substitute for PFOS with large demand and application in the Chinese market. However, little is known about potential developmental effects of OBS. In this study, zebrafish embryos were acutely exposed to different concentrations of OBS and the positive control PFOS for a comparative developmental toxicity assessment. OBS caused hatching delays, body axis curvature, neurobehavioral inhibition and abnormal cardiovascular development. These organismal effects were accompanied by change of development related genes expression profile, in which some cases were similar to PFOS. Overall, the toxic effects induced by OBS were generally milder than that of PFOS. Further investigation suggested that both OBS and PFOS disrupted ciliogenesis, evidenced by the ciliary immunostaining, changes in gene expression of kinesin family, dynein arm family and tubulin family members, as well as downregulation of the abundance of motor proteins including KIF3C, DYNC1H1 and DYNC1LI1. The influence of PFOS was stronger than that of OBS on ciliary genes and proteins. Molecular docking analysis revealed that both OBS and PFOS fitted into the motor proteins tightly, but binding affinity between OBS and motor proteins was lower than PFOS. Collectively, OBS and PFOS may act on ciliary motor proteins to interfere with ciliogenesis, leading to ciliary dysfunction and providing a novel probable action mode linked to developmental toxicity. This raises concerns regarding the health risks of the novel PFOS alternative OBS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping