PUBLICATION
Crosstalk of cholinergic pathway on thyroid disrupting effects of the insecticide chlorpyrifos in zebrafish (Danio rerio)
- Authors
- Qiao, K., Hu, T., Jiang, Y., Huang, J., Hu, J., Gui, W., Ye, Q., Li, S., Zhu, G.
- ID
- ZDB-PUB-201123-8
- Date
- 2020
- Source
- The Science of the total environment 757: 143769 (Journal)
- Registered Authors
- Keywords
- Chlorpyrifos, Crosstalk, Neurotoxicity, Thyroid hormone, Zebrafish
- MeSH Terms
-
- Animals
- Chlorpyrifos*/toxicity
- Cholinergic Agents
- Embryo, Nonmammalian
- Insecticides*/toxicity
- Molecular Docking Simulation
- Thyroid Gland
- Zebrafish
- PubMed
- 33221011 Full text @ Sci. Total Environ.
Citation
Qiao, K., Hu, T., Jiang, Y., Huang, J., Hu, J., Gui, W., Ye, Q., Li, S., Zhu, G. (2020) Crosstalk of cholinergic pathway on thyroid disrupting effects of the insecticide chlorpyrifos in zebrafish (Danio rerio). The Science of the total environment. 757:143769.
Abstract
Chlorpyrifos is a widely used organophosphate insecticide and ubiquitously detected in the environment. However, little attention has been paid to its endocrine disrupting effect to non-target organisms. In the present study, zebrafish was exposed to 13 and 65 μg/L of chlorpyrifos for 7 and 10 days to determine the induced neurotoxicity and the alteration of thyroid metabolism. The 120 h LC50 and LC10 of chlorpyrifos was estimated as 1.35 mg/L and 0.62 mg/L based on the acute embryo toxicity assay, respectively. The acetylcholinesterase (AChE) inhibitory was detected by 13 μg/L chlorpyrifos and could be reversed by the co-exposure of 100 and 1000 μg/L anticholinergic agent atropine. For thyroid hormone level, 13 and 65 μg/L of chlorpyrifos induced increased free T3 levels in 10 dpf (days post-fertilization). The expression of thyroid related genes in 7 and 10 dpf exposed zebrafish were measured by the quantitative Real-Time PCR (qRT-PCR) assay. The mRNA expression of tshba, thrb, crhb, ttr, tpo, ugt1ab and slc5a5 had significant change. However, the alterations of thyroid hormone and mRNA expression could be partly rescued by the addition of atropine. The molecular docking of chlorpyrifos and T3 to the thyroid receptor β in zebrafish using homology modelling and CDOCKER procedures shown weaker binding ability of chlorpyrifos compared to T3. Therefore, we concluded that the disturbance of thyroid signaling in zebrafish might arise from the developmental neurotoxicity induced by chlorpyrifos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping