PUBLICATION
CRISPR/Cas9-induced nos2b mutant zebrafish display behavioral abnormalities
- Authors
- Gao, L., Penglee, R., Huang, Y., Yi, X., Wang, X., Liu, L., Gong, X., Bao, B.
- ID
- ZDB-PUB-201120-152
- Date
- 2020
- Source
- Genes, brain, and behavior 20(5): e12716 (Journal)
- Registered Authors
- Bao, Baolong
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/metabolism
- Gene Deletion
- Homer Scaffolding Proteins/genetics
- Homer Scaffolding Proteins/metabolism
- Motor Activity*
- Mutation
- Nitric Oxide Synthase/genetics*
- Nitric Oxide Synthase/metabolism
- Synaptophysin/genetics
- Synaptophysin/metabolism
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 33200539 Full text @ Genes Brain Behav.
Citation
Gao, L., Penglee, R., Huang, Y., Yi, X., Wang, X., Liu, L., Gong, X., Bao, B. (2020) CRISPR/Cas9-induced nos2b mutant zebrafish display behavioral abnormalities. Genes, brain, and behavior. 20(5):e12716.
Abstract
The immunomodulatory function of nitric oxide synthase (NOS2) has been extensively studied. However, some behavioral abnormalities caused by its mutations have been found in a few rodent studies, of which the molecular mechanism remains elusive. In this research, we generated nos2b gene knockout zebrafish (nos2b sou2/sou2 ) using CRISPR/Cas9 approach and investigated their behavioral and molecular changes by doing a series of behavioral detections, morphological measurements, and molecular analyses. We found that, compared with nos2b +/+ zebrafish, nos2b sou2/sou2 zebrafish exhibited enhanced motor activity; additionally, nos2b sou2/sou2 zebrafish were characterized by smaller brain size, abnormal structure of optic tectum, reduced mRNA level of presynaptic synaptophysin and postsynaptic homer1, and altered response to sodium nitroprusside/methylphenidate hydrochloride treatment. These findings will likely contribute to future studies of behavioral regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping