PUBLICATION
A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes
- Authors
- Chen, X., Xu, J., Suo, F., Yu, C., Zhang, D., Chen, J., Wu, Q., Jing, S., Li, L., Huang, W.
- ID
- ZDB-PUB-201028-11
- Date
- 2020
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 229: 117949 (Journal)
- Registered Authors
- Huang, Wei
- Keywords
- High-throughput, Imaging, Paper-based devices, Point-of-care testing, Zinc ions
- MeSH Terms
-
- Animals
- Cattle
- Fluoresceins/chemistry*
- Fluorescent Dyes/chemistry*
- HeLa Cells
- Hep G2 Cells
- Humans
- Point-of-Care Testing/statistics & numerical data*
- Serum/metabolism*
- Spectrometry, Fluorescence
- Water/metabolism*
- Zebrafish/metabolism*
- Zinc/analysis*
- PubMed
- 31864152 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Chen, X., Xu, J., Suo, F., Yu, C., Zhang, D., Chen, J., Wu, Q., Jing, S., Li, L., Huang, W. (2020) A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 229:117949.
Abstract
The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping