PUBLICATION
Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish
- Authors
- Zheng, Y., Tian, C., Fan, C., Xu, N., Xiao, J., Zhao, X., Lu, Z., Cao, H., Liu, J., Yu, L.
- ID
- ZDB-PUB-201023-12
- Date
- 2020
- Source
- Journal of ethnopharmacology 267: 113497 (Journal)
- Registered Authors
- Keywords
- Anti-inflammatory, LPS, RAW 264.7 cell, Sheng-Mai Yin, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Anti-Inflammatory Agents/pharmacology*
- Copper Sulfate
- Cytokines/metabolism
- Disease Models, Animal
- Drug Combinations
- Drugs, Chinese Herbal/pharmacology*
- Inflammation/chemically induced
- Inflammation/immunology
- Inflammation/metabolism
- Inflammation/prevention & control*
- Inflammation Mediators/metabolism*
- Lipopolysaccharides
- Macrophages/drug effects*
- Macrophages/immunology
- Macrophages/metabolism
- Mice
- NF-KappaB Inhibitor alpha/metabolism
- NF-kappa B/metabolism
- Neutrophil Infiltration/drug effects
- Neutrophils/drug effects*
- Neutrophils/immunology
- Neutrophils/metabolism
- RAW 264.7 Cells
- STAT3 Transcription Factor/metabolism
- Signal Transduction
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 33091492 Full text @ J. Ethnopharmacol.
Citation
Zheng, Y., Tian, C., Fan, C., Xu, N., Xiao, J., Zhao, X., Lu, Z., Cao, H., Liu, J., Yu, L. (2020) Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish. Journal of ethnopharmacology. 267:113497.
Abstract
Ethnopharmacological relevance Sheng-Mai Yin (SMY), a famous traditional Chinese medicine formula, has been commonly used in China for centuries to treat various diseases, such as inflammation-related diseases. However, the anti-inflammatory activity of SMY and its potential mechanisms still have not yet been clearly understood.
Aim of the study In this study, we aimed to determine the anti-inflammatory effect of SMY and explore its underlying mechanisms both on RAW 264.7 cells and zebrafish.
Materials and methods The levels of pro-inflammatory cytokines IL-6 and TNF-α secreted by RAW 264.7 cells were measured by ELISA. The protein expressions of IκBα, p-IκBα (Ser32), STAT3 and p-STAT3 (Tyr705) were determined by Western blotting. And the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 macrophage cells was detected by confocal microscopy. Moreover, the in vivo anti-inflammatory effect of SMY and its potential mechanisms were further investigated by survival analysis, hematoxylin-eosin staining (H&E), observation of neutrophil migration and quantitative real-time PCR (qRT-PCR) analysis in zebrafish inflammatory models.
Results SMY reduced the release of IL-6 and TNF-α, inhibited the phosphorylation of IκBα and STAT3 as well as the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 cells. Furthermore, the increased survival, decreased infiltration of inflammatory cells and the attenuated migration of neutrophils together suggested the in vivo anti-inflammatory effects of SMY. More importantly, SMY reduced the gene expressions of pro-inflammatory cytokines and suppressed LPS-induced up-regulation of NF-κB, IκBα and STAT3 in zebrafish inflammatory models.
Conclusion SMY exerts significant anti-inflammatory effects with a potential mechanism of inhibiting the NF-κB and STAT3 signal pathways. Our findings suggest a scientific rationale of SMY to treat inflammatory diseases in clinic.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping