PUBLICATION
Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3
- Authors
- Liao, G., Zhao, Z., Yang, H., Li, X.
- ID
- ZDB-PUB-201022-3
- Date
- 2020
- Source
- The Journal of international medical research 48: 300060520963993 (Journal)
- Registered Authors
- Keywords
- Honokiol, cyclooxygenase-2, ionizing irradiation, radiation-induced brain injury, reactive oxygen species, sirtuin 3
- MeSH Terms
-
- Animals
- Biphenyl Compounds
- Brain/metabolism
- Brain Injuries*
- Lignans
- Oxidative Stress
- Reactive Oxygen Species
- Sirtuin 3*/genetics
- Sirtuin 3*/metabolism
- Zebrafish/metabolism
- PubMed
- 33081556 Full text @ J Int Med Res
Citation
Liao, G., Zhao, Z., Yang, H., Li, X. (2020) Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3. The Journal of international medical research. 48:300060520963993.
Abstract
Objective Sirtuin 3 (SIRT3) plays a vital role in regulating oxidative stress in tissue injury. The aim of this study was to evaluate the radioprotective effects of honokiol (HKL) in a zebrafish model of radiation-induced brain injury and in HT22 cells.
Methods The levels of reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were evaluated in the zebrafish brain and HT22 cells. The expression levels of SIRT3 and cyclooxygenase-2 (COX-2) were measured using western blot assays and real-time polymerase chain reaction (RT-PCR).
Results HKL treatment attenuated the levels of ROS, TNF-α, and IL-1β in both the in vivo and in vitro models of irradiation injury. Furthermore, HKL treatment increased the expression of SIRT3 and decreased the expression of COX-2. The radioprotective effects of HKL were achieved via SIRT3 activation.
Conclusions HKL attenuated oxidative stress and pro-inflammatory responses in a SIRT3-dependent manner in radiation-induced brain injury.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping