Regulation of Zebrafish Fin Regeneration by Vitamin D Signaling
- Chen, A., Han, Y., Poss, K.D.
- Developmental Dynamics : an official publication of the American Association of Anatomists 250(9): 1330-1339 (Journal)
- Registered Authors
- Chen, Anzhi, Han, Yanchao, Poss, Kenneth D.
- fins, regeneration, tissue regeneration enhancer elements, vitamin D, zebrafish
- MeSH Terms
- Animal Fins/metabolism
- Animals, Genetically Modified
- Vitamin D*/metabolism
- Vitamin D*/pharmacology
- Zebrafish Proteins/metabolism
- 33064344 Full text @ Dev. Dyn.
Chen, A., Han, Y., Poss, K.D. (2020) Regulation of Zebrafish Fin Regeneration by Vitamin D Signaling. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(9):1330-1339.
Background Vitamin D is an essential nutrient that has long been known to regulate skeletal growth and integrity. In models of major appendage regeneration, treatment with vitamin D analogs has been reported to improve aspects of zebrafish fin regeneration in specific disease or gene misexpression contexts, but also to disrupt pattern in regenerating salamander limbs. Recently, we reported strong mitogenic roles for vitamin D signaling in several zebrafish tissues throughout life stages, including epidermal cells and osteoblasts of adult fins. To our knowledge, molecular genetic approaches to dissect vitamin D function in appendage regeneration have not been described.
Results Using a knock-in GFP reporter for the expression of the vitamin D target gene and negative regulator cyp24a1, we identified active vitamin D signaling in adult zebrafish fins during tissue homeostasis and regeneration. Transgenic expression of cyp24a1 or a dominant-negative vitamin D receptor (VDR) inhibited regeneration of amputated fins, whereas global vitamin D treatment accelerated regeneration. Using tissue regeneration enhancer elements, we found that local enhancement of VDR expression could improve regeneration with low doses of a vitamin D analog.
Conclusions Vitamin D signaling enhances the efficacy of fin regeneration in zebrafish. This article is protected by copyright. All rights reserved.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes