PUBLICATION
Impact of Pronase, Sodium Thiosulfate, and Methylene Blue Combinations on Development and Survival of Sodium Hypochlorite Surface-Disinfected Zebrafish (Danio rerio) Embryos
- Authors
- Peneyra, S.M., Lerpiriyapong, K., Riedel, E.R., Lipman, N.S., Lieggi, C.
- ID
- ZDB-PUB-201015-16
- Date
- 2020
- Source
- Zebrafish 17: 342-353 (Journal)
- Registered Authors
- Keywords
- embryo disinfection, methylene blue, pronase, sodium hypochlorite, sodium thiosulfate, zebrafish
- MeSH Terms
-
- Animals
- Disinfectants/adverse effects*
- Disinfection
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology
- Embryonic Development/drug effects*
- Methylene Blue/adverse effects*
- Pronase/adverse effects*
- Sodium Hypochlorite/adverse effects*
- Thiosulfates/adverse effects*
- Zebrafish/embryology
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 33048660 Full text @ Zebrafish
Citation
Peneyra, S.M., Lerpiriyapong, K., Riedel, E.R., Lipman, N.S., Lieggi, C. (2020) Impact of Pronase, Sodium Thiosulfate, and Methylene Blue Combinations on Development and Survival of Sodium Hypochlorite Surface-Disinfected Zebrafish (Danio rerio) Embryos. Zebrafish. 17:342-353.
Abstract
Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium thiosulfate, and/or methylene blue; however, the impact of these chemicals on embryo survival and development has not been evaluated. In this study, AB and casper embryos were exposed to disinfection protocols that used Pron, sodium thiosulfate, and/or methylene blue (given alone, in various combinations, or all three combined) with 50 and 100 ppm sodium hypochlorite performed 6 and 24 h postfertilization (HPF). All groups were evaluated for survival, hatching, and malformations at 5 days postfertilization. Maximal survival (69%-97%) and hatching rates (66%-94%) were generally observed with sodium hypochlorite disinfection followed by exposure to both Pron and sodium thiosulfate and maintenance in standard embryo medium without methylene blue. Methylene blue had variable effects on survival and hatching. Higher survival and hatching rates were seen in AB embryos disinfected at 6 HPF and casper embryos disinfected at 24 HPF. Susceptibility to sodium hypochlorite toxicity differed by strain, emphasizing the need to test disinfection protocols on small embryo cohorts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping