PUBLICATION
Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species
- Authors
- Ji, S., Gong, Q., Zhang, W., Zheng, J., Peng, B., Yang, M.
- ID
- ZDB-PUB-201011-14
- Date
- 2020
- Source
- Fish & shellfish immunology 107(Pt A): 64-72 (Journal)
- Registered Authors
- Keywords
- Bacterial ghosts, Immunoprotection, Polyvalent vaccine, Vibrio parahaemolyticus
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/metabolism
- Anti-Bacterial Agents/pharmacology*
- Bacterial Vaccines/genetics
- Bacterial Vaccines/metabolism
- Bacterial Vaccines/pharmacology*
- Fish Diseases/immunology
- Fish Diseases/microbiology
- Fish Diseases/prevention & control*
- Vibrio Infections/immunology
- Vibrio Infections/microbiology
- Vibrio Infections/prevention & control
- Vibrio Infections/veterinary*
- Vibrio parahaemolyticus/immunology*
- Zebrafish*
- PubMed
- 33038509 Full text @ Fish Shellfish Immunol.
Citation
Ji, S., Gong, Q., Zhang, W., Zheng, J., Peng, B., Yang, M. (2020) Recombinant Vibrio parahaemolyticus ghosts protect zebrafish against infection by Vibrio species. Fish & shellfish immunology. 107(Pt A):64-72.
Abstract
Aquatic animals are frequently threated by bacterial pathogens. The most economic and efficient protection against bacterial infection are through vaccine immunization. The various serotypes of the pathogens, such as Vibrios, hurdle the development of the vaccines, especially polyvalent vaccines. Here, we demonstrate that recombinant bacterial ghost is a good candidate for multivalent vaccine. By expressing PhiX174 gene E alone or co-expressing the gene E with two genes encoding outer membrane proteins (VP1667 and VP2369) in V. parahaemolyticus, we generated the recombinant V. parahaemolyticus ghosts VPG and rVPGs respectively. Fish immunized with either VPG or rVPG showed increased survival against the infection by either V. parahaemolyticus or V. alginolyticus, with a better protective effect by immunization with rVPG. Our furthermore studies show that rVPG stimulates stronger innate immune responses by increasing the expression of tnfα, il1β, il6, il8 and il10 as well as that of c3b, lyz, and tlr5, the key players linking the innate and adaptive immune responses upon microbial stimulation. In summary, VPG and rVPG can protect zebrafish against the infection from at least two Vibrio species, suggesting its potential value for further aquaculture vaccines development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping