PUBLICATION
Identification of the 14-3-3 β/α-A protein as a novel maternal peptidoglycan-binding protein that protects embryos of zebrafish against bacterial infections
- Authors
- Wang, X., Ren, Y., Li, J., Ji, Z., Chen, F., Wang, X.
- ID
- ZDB-PUB-201002-66
- Date
- 2020
- Source
- Developmental and comparative immunology 114: 103867 (Journal)
- Registered Authors
- Keywords
- 14-3-3 proteins, Antibacterial activity, Maternal immunity
- MeSH Terms
-
- 14-3-3 Proteins/genetics
- 14-3-3 Proteins/metabolism*
- Animals
- Antibodies, Blocking/metabolism
- Bacterial Infections/microbiology
- Bacterial Infections/veterinary*
- Disease Resistance
- Embryo, Nonmammalian/immunology
- Embryo, Nonmammalian/microbiology
- Fish Diseases/immunology*
- Fish Diseases/microbiology*
- Immunity, Maternally-Acquired
- Peptidoglycan/metabolism*
- Protein Binding
- Receptors, Pattern Recognition/genetics
- Receptors, Pattern Recognition/metabolism*
- Zebrafish/immunology*
- Zebrafish/microbiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32931839 Full text @ Dev. Comp. Immunol.
Citation
Wang, X., Ren, Y., Li, J., Ji, Z., Chen, F., Wang, X. (2020) Identification of the 14-3-3 β/α-A protein as a novel maternal peptidoglycan-binding protein that protects embryos of zebrafish against bacterial infections. Developmental and comparative immunology. 114:103867.
Abstract
14-3-3 proteins are widespread in animals, but their functions and mechanisms remain poorly defined. Here we clearly demonstrate that 14-3-3 β/α-A is a newly identified PGN-binding protein present abundantly in the eggs/embryos of zebrafish. We also show that recombinant 14-3-3 β/α-A acts as a pattern recognition receptor capable of identifying the bacterial signature molecule PGN, binding the bacteria, and functions as an antibacterial effector molecule directly killing the bacteria. Importantly, microinjection of r14-3-3 β/α-A into early embryos significantly enhanced the resistance of the embryos against pathogenic A. hydrophila challenge, and this enhanced bacterial resistance was markedly reduced by co-injection of anti-14-3-3 β/α-A antibody. Collectively, these results indicate that 14-3-3 β/α-A is a maternal PGN-binding protein that can protect the early embryos of zebrafish against pathogenic attacks, a novel role assigned to 14-3-3 β/α-A proteins. This work also provides new insights into 14-3-3 proteins that are widely distributed in various animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping