PUBLICATION
Red elemental selenium (Se0 ) improves the immunoactivities of EPC cells, crucian carp and zebrafish against spring viremia of carp virus (SVCV)
- Authors
- Tian, J., Zhang, Y., Zhu, R., Wu, Y., Liu, X., Wang, X.
- ID
- ZDB-PUB-201002-224
- Date
- 2020
- Source
- Journal of Fish Biology 98(1): 208-218 (Journal)
- Registered Authors
- Keywords
- Antiviral activity, Crucian carp, Red elemental selenium, SVCV infection, Zebrafish
- MeSH Terms
-
- Adjuvants, Immunologic/pharmacology
- Adjuvants, Immunologic/therapeutic use
- Animals
- Antiviral Agents/pharmacology
- Carcinoma
- Carps/immunology*
- Carps/virology
- Cells, Cultured
- Fish Diseases/drug therapy*
- Fish Diseases/immunology*
- Gene Expression Regulation/drug effects
- Interferons/genetics
- Rhabdoviridae
- Rhabdoviridae Infections/drug therapy
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary*
- Selenium/pharmacology*
- Selenium/therapeutic use
- Viremia/drug therapy
- Viremia/immunology
- Viremia/veterinary*
- Zebrafish/immunology*
- Zebrafish/virology
- PubMed
- 33000466 Full text @ J. Fish Biol.
Citation
Tian, J., Zhang, Y., Zhu, R., Wu, Y., Liu, X., Wang, X. (2020) Red elemental selenium (Se0 ) improves the immunoactivities of EPC cells, crucian carp and zebrafish against spring viremia of carp virus (SVCV). Journal of Fish Biology. 98(1):208-218.
Abstract
Selenium, as an essential trace element, interferes through selenoproteins in many physiological processes of plants and mammals. Its antiviral activity has recently attracted more attention since selenium improves the antiviral capacity of animal cells against a few viruses relevant to human diseases. In this study, the red elemental selenium was purified from the fermentation of Herbaspirillum camelliae WT00C and then used to culture EPC cells or feed crucian carp and zebrafish. Finally, its antiviral effects were respectively investigated at the levels of cell and living fishes after SVCV infection. At the cell level, 5, 10, 20 μg/ml red elemental selenium significantly induced the expression of IFN and ISG15 genes in EPC cells. The viral TCID50 values in the EPC cells incubated with 5, 10, 20 μg/ml red elemental selenium were significantly less than those of the control. More expression of IFN and ISG15 genes and less TCID50 values indicate that red elemental selenium indeed improves the antiviral capability of EPC cells. In the crucian carp fed with the food containing 5 and 10 μg/g red elemental selenium, IFN expressions showed 13- and 39-fold increases. at the 16th day of post-injection, and its expression was dependent on selenium concentrations. Meanwhile, no fish death occurred in all experimental groups. In the zebrafish fed with the red worm containing 5 μg/g red elemental selenium, IFN and Mx expressions and survival rate were significantly higher than those of the control. Our results demonstrate that red elemental selenium indeed improves the antiviral activity of fish. The antiviral effects of selenium mainly come from its immune regulation through its incorporation into selenoproteins. The properly selenium is contributive to improving fish immunity, whereas excess selenium causes excessive immune and inflammatory responses. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping