PUBLICATION
First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period
- Authors
- Roche, J., Hergalant, S., Depp, A., Ben Ammar, I., Lafont, A.G., Policar, T., Fontaine, P., Milla, S.
- ID
- ZDB-PUB-201002-196
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Part D, Genomics & proteomics 36: 100747 (Journal)
- Registered Authors
- Keywords
- Dopamine, Pikeperch, RNA-sequencing, de novo transcriptome assembly
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Female
- Gene Expression Regulation, Developmental*
- Ovulation*
- Perches/genetics
- Perches/metabolism*
- Phylogeny
- RNA-Seq
- Receptors, Dopamine/genetics
- Receptors, Dopamine/metabolism*
- Transcriptome*
- PubMed
- 32987329 Full text @ Comp. Biochem. Physiol. D Genom. Prot.
Citation
Roche, J., Hergalant, S., Depp, A., Ben Ammar, I., Lafont, A.G., Policar, T., Fontaine, P., Milla, S. (2020) First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period. Comparative biochemistry and physiology. Part D, Genomics & proteomics. 36:100747.
Abstract
Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D1 (former D1A), D5 (former D1B), D6 (former D1C and D1D) and D7 (former D1E), while the D2 family encloses five subtypes, D2, D3, D4, D8 (former D2like or D2l) and D9 (former D4-related sequence or D4-rs). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D1 family: two D1, one D5a, one D6a and one D6b and to the D2 family: two spliced variants of D2, one D3, one D8 and one D9. Unlike zebrafish, the subtypes D4 and D7 have not yet been isolated in pikeperch. As expected D1, D3, D8 and D9 are mostly expressed in brain parts except for the cerebellum (D1 and D3). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping