PUBLICATION
Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish
- Authors
- Huckenpahler, A.L., Lookfong, N.A., Warr, E., Heffernan, E., Carroll, J., Collery, R.F.
- ID
- ZDB-PUB-201002-188
- Date
- 2020
- Source
- Translational vision science & technology 9: 18 (Journal)
- Registered Authors
- Collery, Ross
- Keywords
- Müller cell, OCT, cone photoreceptor, regeneration, zebrafish
- MeSH Terms
-
- Animals
- Diagnostic Tests, Routine
- Regeneration
- Retinal Cone Photoreceptor Cells*
- Tomography, Optical Coherence
- Zebrafish*
- PubMed
- 32983626 Full text @ Transl Vis Sci Technol
Citation
Huckenpahler, A.L., Lookfong, N.A., Warr, E., Heffernan, E., Carroll, J., Collery, R.F. (2020) Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish. Translational vision science & technology. 9:18.
Abstract
Purpose To observe and characterize cone degeneration and regeneration in a selective metronidazole-mediated ablation model of ultraviolet-sensitive (UV) cones in zebrafish using in vivo optical coherence tomography (OCT) imaging.
Methods Twenty-six sws1:nfsB-mCherry;sws2:eGFP zebrafish were imaged with OCT, treated with metronidazole to selectively kill UV cones, and imaged at 1, 3, 7, 14, 28, or 56 days after ablation. Regions 200 × 200 µm were cropped from volume OCT scans to count individual UV cones before and after ablation. Fish eyes were fixed, and immunofluorescence staining was used to corroborate cone density measured from OCT and to track monocyte response.
Results Histology shows significant loss of UV cones after metronidazole treatment with a slight increase in observable blue cone density one day after treatment (Kruskal, Wallis, P = 0.0061) and no significant change in blue cones at all other timepoints. Regenerated UV cones measured from OCT show significantly lower density than pre-cone-ablation at 14, 28, and 56 days after ablation (analysis of variance, P < 0.01, P < 0.0001, P < 0.0001, respectively, 15.9% of expected nonablated levels). Histology shows significant changes to monocyte morphology (mixed-effects analysis, P < 0.0001) and retinal position (mixed-effects analysis, P < 0.0001).
Conclusions OCT can be used to observe loss of individual cones selectively ablated by metronidazole prodrug activation and to quantify UV cone loss and regeneration in zebrafish. OCT images also show transient changes to the blue cone mosaic and inner retinal layers that occur concomitantly with selective UV cone ablation.
Translational relevance Profiling cone degeneration and regeneration using in vivo imaging enables experiments that may lead to a better understanding of cone regeneration in vertebrates.
Errata / Notes
This article is corrected by ZDB-PUB-220906-228 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping