PUBLICATION
Role of GnRH and GnIH in paracrine/autocrine control of final oocyte maturation
- Authors
- Fallah, H.P., Habibi, H.R.
- ID
- ZDB-PUB-201002-108
- Date
- 2020
- Source
- General and comparative endocrinology 299: 113619 (Journal)
- Registered Authors
- Keywords
- Caspase-3 activity, Germinal vesicle breakdown (GVB), Gonadotropin-inhibitory hormone (GnIH), Gonadotropin-releasing hormone (GnRH), Human chorionic gonadotropin (hCG)
- MeSH Terms
-
- Animals
- Autocrine Communication*
- Female
- Gonadotropin-Releasing Hormone/pharmacology*
- Hypothalamic Hormones/pharmacology*
- Meiosis*
- Oocytes/cytology*
- Oocytes/drug effects
- Oocytes/metabolism
- Ovarian Follicle/cytology*
- Ovarian Follicle/drug effects
- Ovarian Follicle/metabolism
- Paracrine Communication*
- Zebrafish
- PubMed
- 32956700 Full text @ Gen. Comp. Endocrinol.
Citation
Fallah, H.P., Habibi, H.R. (2020) Role of GnRH and GnIH in paracrine/autocrine control of final oocyte maturation. General and comparative endocrinology. 299:113619.
Abstract
The control of oocyte growth and its final maturation is multifactorial and involves a number of hypothalamic, hypophyseal, and peripheral hormones. In this study, we investigated the direct actions of the gonadotropin-releasing hormone (GnRH) and the gonadotropin-inhibitory hormone (GnIH), which are expressed in the ovarian follicles, on final oocyte maturation in zebrafish, in vitro. Our study demonstrates the expression of GnRH and GnIH in the ovarian follicles of zebrafish (Danio rerio) at different stages of development and provides information on the direct action of these hormones on final oocyte maturation. Treatment with both GnRH and GnIH peptides stimulated the germinal vesicle breakdown (GVBD) of the late-vitellogenic oocyte. Both the GnRH and GnIH treatments showed no significant change in the caspase-3 activity of pre-vitellogenic and mid-vitellogenic oocytes, while they displayed different responses in the late-vitellogenic follicles. The GnRH treatment increased caspase-3 activity, whereas the GnIH reduced caspase-3 activity in the late-vitellogenic follicles. We also investigated the effects of GnRH and GnIH on the hCG-induced resumption of meiosis and caspase activity in vitro. GnRH and GnIH were found to have a similar effect on the hCG-induced resumption of meiosis, while they showed the opposite effect on caspase-3 activity. Furthermore, we investigated the effects of concomitant treatment of GnRH and GnIH peptides with hCG. The results demonstrated that the presence of both GnRH3 and GnIH are necessary for the normal induction of final oocyte maturation by gonadotropins. The findings support the hypothesis that GnIH and GnRH peptides produced in the ovary are part of a complex multifactorial regulatory system that controls zebrafish final oocyte maturation in paracrine/autocrine manner working in concert with gonadotropin hormones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping