PUBLICATION
Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation
- Authors
- Moraleda-Prados, J., Caballero-Huertas, M., Valdivieso, A., Joly, S., Ji, J., Roher, N., Ribas, L.
- ID
- ZDB-PUB-200906-8
- Date
- 2020
- Source
- Developmental and comparative immunology 114: 103848 (Journal)
- Registered Authors
- Keywords
- LPS, gonads, immune, methylation, reproduction, sex ratio, zebrafish
- Datasets
- GEO:GSE134400
- MeSH Terms
-
- Animals
- Caspase 9/genetics
- Caspase 9/metabolism
- CpG Islands/genetics
- DNA Methylation
- Epigenesis, Genetic
- Gene Expression Profiling
- Genetic Association Studies
- Gonads/metabolism*
- Immunity
- Immunity, Innate
- Immunization
- Interleukin-1beta/genetics
- Interleukin-1beta/metabolism
- Lipopolysaccharides/immunology
- Lipopolysaccharides/metabolism*
- Pseudomonas aeruginosa/metabolism*
- Reproduction
- Sex Differentiation
- Sex Ratio
- Zebrafish/immunology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32888969 Full text @ Dev. Comp. Immunol.
Citation
Moraleda-Prados, J., Caballero-Huertas, M., Valdivieso, A., Joly, S., Ji, J., Roher, N., Ribas, L. (2020) Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation. Developmental and comparative immunology. 114:103848.
Abstract
Infections are able to trigger epigenetic modifications; however, epigenetic-mediating infections in the immune system in fish is currently unavailable. Within this purpose, zebrafish were immune-stimulated with three lipopolysaccharides (LPS) during sex differentiation. Methylation patterns of three immune genes were studied by a candidate gene approach together with gene expression analysis, and in adulthood, sex ratios were determined. It was shown that the entrance of LPS was through the gills and accumulated in the pronephros. Significant hypomethylation levels of CASP9 and a significant CpG site for IL1β after Pseudomonas aeruginosa LPS exposure were found. No methylation difference was observed for TNFα. Gene expression and correlation data differed among studied genes. Sex ratios showed a feminization in dose and LPS strain-dependent manner. Here, it is provided epigenetic regulatory mechanisms derived by innate response and the first evidence of possible epigenetic interactions between the immune and reproductive systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping