PUBLICATION
Exposure to a high dose of amoxicillin causes behavioral changes and oxidative stress in young zebrafish
- Authors
- Gonçalves, C.L., Vasconcelos, F.F.P., Wessler, L.B., Lemos, I.S., Candiotto, G., Lin, J., Matias, M.B.D., Rico, E.P., Streck, E.L.
- ID
- ZDB-PUB-200904-8
- Date
- 2020
- Source
- Metabolic Brain Disease 35(8): 1407-1416 (Journal)
- Registered Authors
- Keywords
- Amoxicillin, Autism, Oxidative stress, Zebrafish
- MeSH Terms
-
- Age Factors
- Amoxicillin/administration & dosage
- Amoxicillin/toxicity*
- Animals
- Anti-Bacterial Agents/administration & dosage
- Anti-Bacterial Agents/toxicity*
- Dose-Response Relationship, Drug
- Lipid Peroxidation/drug effects*
- Lipid Peroxidation/physiology
- Oxidative Stress/drug effects*
- Oxidative Stress/physiology
- Social Interaction/drug effects*
- Zebrafish
- PubMed
- 32876824 Full text @ Metab. Brain Dis.
Citation
Gonçalves, C.L., Vasconcelos, F.F.P., Wessler, L.B., Lemos, I.S., Candiotto, G., Lin, J., Matias, M.B.D., Rico, E.P., Streck, E.L. (2020) Exposure to a high dose of amoxicillin causes behavioral changes and oxidative stress in young zebrafish. Metabolic Brain Disease. 35(8):1407-1416.
Abstract
Autistic spectrum disorder (ASD) is a group of early-onset neurodevelopmental disorders characterized by impaired social and communication skills. Autism is widely described as a behavioral syndrome with multiple etiologies where may exhibit neurobiological, genetic, and psychological deficits. Studies have indicated that long term use of antibiotics can alter the intestinal flora followed by neuroendocrine changes, leading to behavioral changes. Indeed, previous studies demonstrate that a high dose of amoxicillin can change behavioral parameters in murine animal models. The objective was to evaluate behavioral and oxidative stress parameters in zebrafish exposed to a high dose of amoxicillin for 7 days. Young zebrafish were exposed to a daily concentration of amoxicillin (100 mg/L) for 7 days. Subsequently, the behavioral analysis was performed, and the brain content was dissected for the evaluation of oxidative stress parameters. Zebrafish exposed to a high dose of amoxicillin showed locomotor alteration and decreased social interaction behavior. In addition, besides the significant decrease of sulfhydryl content, there was a marked decrease in catalase activity, as well as an increased superoxide dismutase activity in brain tissue. Thus, through the zebrafish model was possible to note a central effect related to the exposition of amoxicillin, the same as observed in murine models. Further, the present data reinforce the relation of the gut-brain-axis and the use of zebrafish as a useful tool to investigate new therapies for autistic traits.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping