ZFIN ID: ZDB-PUB-200901-8
Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish
Ozdemir, Y.I., Hansen, C.A., Ramy, M.A., Troconis, E.L., McNeil, L.D., Trapani, J.G.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2191: 201-220 (Chapter)
Registered Authors: Trapani, Josef
Keywords: Behavioral kinematics, Escape responses, Field potentials, Locomotion, Mauthner cells, Optophysiology, Startle reflexes
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • Channelrhodopsins/genetics*
  • Channelrhodopsins/physiology
  • Evoked Potentials/genetics*
  • Evoked Potentials/physiology
  • Hair Cells, Auditory/metabolism
  • Larva/physiology
  • Locomotion/genetics
  • Locomotion/physiology
  • Neurons/metabolism*
  • Neurons/pathology
  • Optogenetics/methods*
  • Reflex, Startle/physiology
  • Swimming/physiology
  • Zebrafish/genetics
  • Zebrafish/physiology
PubMed: 32865747 Full text @ Meth. Mol. Biol.
ABSTRACT
Zebrafish are an excellent model organism to study many aspects of vertebrate sensory encoding and behavior. Their escape responses begin with a C-shaped body bend followed by several swimming bouts away from the potentially threatening stimulus. This highly stereotyped motor behavior provides a model for studying startle reflexes and the neural circuitry underlying multisensory encoding and locomotion. Channelrhodopsin (ChR2) can be expressed in the lateral line and ear hair cells of zebrafish and can be excited in vivo to elicit these rapid forms of escape. Here we review our methods for studying transgenic ChR2-expressing zebrafish larvae, including screening for positive expression of ChR2 and recording field potentials and high-speed videos of optically evoked escape responses. We also highlight important features of the acquired data and provide a brief review of other zebrafish research that utilizes or has the potential to benefit from ChR2 and optogenetics.
ADDITIONAL INFORMATION No data available