PUBLICATION
UPLC-TOF-MS/MS metabolomics analysis of zebrafish metabolism by spirotetramat
- Authors
- Zhang, J., Qian, L., Wang, C., Teng, M., Duan, M., Chen, X., Li, X., Wang, C.
- ID
- ZDB-PUB-200818-2
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 266: 115310 (Journal)
- Registered Authors
- Keywords
- Energy metabolism, Mitochondrion, Spirotetramat, Untargeted metabolomics, Zebrafish embryo
- MeSH Terms
-
- Animals
- Aza Compounds
- Metabolomics
- Spiro Compounds
- Tandem Mass Spectrometry
- Water Pollutants, Chemical*
- Zebrafish*
- PubMed
- 32798906 Full text @ Environ. Pollut.
Citation
Zhang, J., Qian, L., Wang, C., Teng, M., Duan, M., Chen, X., Li, X., Wang, C. (2020) UPLC-TOF-MS/MS metabolomics analysis of zebrafish metabolism by spirotetramat. Environmental pollution (Barking, Essex : 1987). 266:115310.
Abstract
Spirotetramat, a member of tetronic and tetramic acid derivatives, is a unique insecticide and acaricide. Although the effect on zebrafish embryos lipid biosynthesis of spirotetramat has been characterized, the energy metabolism and toxic effect mechanism warrant further investigation. To investigate the toxic mechanism of spirotetramat on energy metabolism, zebrafish embryos were exposed to 100, 500 and 1000 µg/L of spirotetramat for 4 days. Untargeted metabolomics showed the synthesis and degradation of ketone pathway metabolites (R)-3-Hydroxybutyric acid and Acetoacetate significantly decreased, as well as increasing the abundance of Anti-Acetyl Coenzyme A Carboxylase protein (ACC1). Down-regulation of the genes related to ß-oxidation and the tricarboxylic acid cycle in the embryos show decreased energy metabolism. Carnitine palmitoyltransferase 1 (CPT- I) significantly decreased while citrate synthase (CS) significantly increased. Additionally, mitochondrial lesions in embryos were found using electron microscopy. Our study provides novel and robust perspectives, which show that spirotetramat treatment in embryos leads to metabolic disturbances that adversely affect cellular energy homeostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping