PUBLICATION
G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio)
- Authors
- Liu, S., Chen, F., Zhang, Y., Cai, L., WenhuiQiu, ., Yang, M.
- ID
- ZDB-PUB-200814-14
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 239: 108868 (Journal)
- Registered Authors
- Keywords
- Estrogen effect, GPER1, Red common carp, mRNA expression
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Carps/metabolism*
- Cells, Cultured
- Estrogens, Non-Steroidal/toxicity*
- Macrophages/cytology
- Macrophages/metabolism
- Phenols/toxicity*
- Receptors, G-Protein-Coupled/physiology*
- Water Pollutants, Chemical/toxicity*
- Water Pollution, Chemical
- PubMed
- 32791253 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Liu, S., Chen, F., Zhang, Y., Cai, L., WenhuiQiu, ., Yang, M. (2020) G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 239:108868.
Abstract
G protein-coupled estrogen receptor 1 (GPER1) plays a crucial role in the regulation of non-genomic estrogen effect. However, the research about fish GPER1 is still limited. The present study aims to obtain the full-length sequence of gper1 from red common carp (Cyprinus carpio) and characterize its expression pattern, and to further explore its potential role in regulating the environmental estrogen induced immunotoxicity. We first cloned the full-length mRNA and genomic sequences of gper1 in C. carpio by PCR, and obtained a 1908 bp sequence with a 1062 bp open reading frame encoding GPER1 protein with 353 amino acids. Additionally, qRT-PCR showed that gper1 was expressed across different tissues in C. carpio, with the highest expression in the brain, which is similar to that in zebrafish. Moreover, applying a luciferase reporter system, we found that the promotor sequence of gper1 has strong activity, and similar to GPER1 in other animals, carp GPER1 also has seven-transmembrane domains, indicating its potential functions. We confirmed the binding ability of GPER1 with G1 and G15 in primary macrophages of C. carpio by testing the related gene expression levels after 6-h exposure, and similar to G1, bisphenol A (BPA), a typical environmental estrogen, could interact with GPER1 to increase the Ca2+ concentration in macrophages treated for 30-min. Furthermore, inhibition of GPER1 with GPER1 antagonist G36 rescued the cellular immunotoxicity caused by BPA, which further suggested that carp GPER1 could mediate the estrogen effect. Our findings contribute to better understanding of the role of carp GPER1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping