PUBLICATION
Pharmacological Validation of the Prepulse Inhibition of Startle Response in Larval Zebrafish using a Commercial Automated System and Software
- Authors
- Banono, N.S., Esguerra, C.V.
- ID
- ZDB-PUB-200728-35
- Date
- 2020
- Source
- Journal of visualized experiments : JoVE (161): (Journal)
- Registered Authors
- Banono, Nancy, Esguerra, Camila V.
- Keywords
- none
- MeSH Terms
-
- Animals
- Larva/drug effects*
- Male
- Prepulse Inhibition/drug effects*
- Reflex, Startle/physiology*
- Reproducibility of Results
- Zebrafish
- PubMed
- 32716380 Full text @ J. Vis. Exp.
Citation
Banono, N.S., Esguerra, C.V. (2020) Pharmacological Validation of the Prepulse Inhibition of Startle Response in Larval Zebrafish using a Commercial Automated System and Software. Journal of visualized experiments : JoVE. (161):.
Abstract
While there is an abundance of commercial and standardized automated systems and software for performing the prepulse inhibition (PPI) assay in rodents, to the best of our knowledge, all PPI assays performed in the zebrafish have, until now, been done using custom made systems which were only available to individual groups. This has thereby presented challenges, particularly with regard to issues of data reproducibility and standardization. In the present work, we generated a protocol that utilizes commercially available automated systems to pharmacologically validate the PPI assay in larval zebrafish. Consistent with published findings, we were able to replicate the results of apomorphine, haloperidol and ketamine on the PPI response of 6 days post-fertilization zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping