|ZFIN ID: ZDB-PUB-200724-21|
Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes
Wang, M., Du, L., Lee, A.C., Li, Y., Qin, H., He, J.
|Source:||The Journal of cell biology 219(9): (Journal)|
|Registered Authors:||Du, Lei, He, Jie, Li, Yan, Qin, Huiwen, Wang, Mei|
|PubMed:||32699896 Full text @ J. Cell Biol.|
Wang, M., Du, L., Lee, A.C., Li, Y., Qin, H., He, J. (2020) Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes. The Journal of cell biology. 219(9):.
ABSTRACTHow astounding neuronal diversity arises from variable cell lineages in vertebrates remains mostly elusive. By in vivo lineage tracing of ∼1,000 single zebrafish retinal progenitors, we identified a repertoire of subtype-specific stereotyped neurogenic lineages. Remarkably, within these stereotyped lineages, GABAergic amacrine cells were born with photoreceptor cells, whereas glycinergic amacrine cells were born with OFF bipolar cells. More interestingly, post-mitotic differentiation blockage of GABAergic and glycinergic amacrine cells resulted in their respecification into photoreceptor and bipolar cells, respectively, suggesting lineage constraint in cell subtype specification. Using single-cell RNA-seq and ATAC-seq analyses, we further identified lineage-specific progenitors, each defined by specific transcription factors that exhibited characteristic chromatin accessibility dynamics. Finally, single pro-neural factors could specify different neuron types/subtypes in a lineage-dependent manner. Our findings reveal the importance of lineage context in defining neuronal subtypes and provide a demonstration of in vivo lineage-dependent induction of unique retinal neuron subtypes for treatment purposes.