ZFIN ID: ZDB-PUB-200708-9
Metabolite profiling of Javanese ginger Zingiber purpureum and identification of antiseizure metabolites via a low-cost open-source zebrafish bioassay-guided isolation
Brillatz, T.E.O., Kubo, M., Takahashi, S., Jozukuri, N., Takechi, K., Queiroz, E.F., Marcourt, L., Allard, P.M., Fish, R., Harada, K., Ishizawa, K., Crawford, A.D., Fukuyama, Y., Wolfender, J.L.
Date: 2020
Source: Journal of Agricultural and Food Chemistry   68(30): 7904-7915 (Journal)
Registered Authors: Fish, Richard
Keywords: none
MeSH Terms:
  • Animals
  • Biological Assay/economics*
  • Disease Models, Animal
  • Ginger/chemistry*
  • Ginger/metabolism
  • Humans
  • Male
  • Mice
  • Mice, Inbred ICR
  • Plant Extracts/administration & dosage*
  • Plant Extracts/chemistry
  • Plant Extracts/metabolism*
  • Seizures/drug therapy*
  • Seizures/metabolism
  • Zebrafish
PubMed: 32628839 Full text @ J. Agric. Food Chem.
The rhizomes of Zingiber purpureum, "Bangle", were investigated for its antiseizure properties using a streamlined and cost-effective zebrafish screening strategy and a mouse epilepsy assay. Its hexane extract demonstrated strong antiseizure activity in zebrafish epilepsy assay and was, therefore, selected for bioactivity-guided fractionation. Twelve compounds (1-12) were isolated and two bioactive phenylbutenoids, trans- (11) and cis-banglene (12), reduced up to 70% of pentylenetetrazole (PTZ)-induced seizures. These compounds showed moderate activity against PTZ-induced seizures in a mouse epilepsy assay. To understand the specificity of Z. purpureum active compounds, its chemical profile was compared to the one of Z. officinale. Their composition was assessed by differential metabolite profiling visualized by molecular network, which revealed only vanillin derivatives and terpenoids as common metabolites and gave a comprehensive view of Z. purpureum composition. This study demonstrates the efficacy of a streamlined zebrafish epilepsy assay, which is therefore suitable for routine screening in phytochemistry laboratories.